Foreign Animal Disease Division, Animal and Plant Quarantine Agency, Gimcheon 39660, Republic of Korea.
College of Veterinary Medicine, Chonnam National University, Gwangju 61186, Republic of Korea.
Viruses. 2023 Jul 14;15(7):1552. doi: 10.3390/v15071552.
Fifteen pig farms were affected by African swine fever (ASF) in South Korea during the outbreaks between 2022 and April 2023. The ASF virus (ASFV) genome was directly extracted from the blood and tissue samples of 15 ASFV-positive pig farms to analyze the genetic characteristics. Phylogenetic analysis revealed that the 15 strains belonged to p72 genotype II and CD2v serogroup 8, which were the central variable region (CVR) I variants of the gene. Fourteen strains were intergenic region (IGR) II variants, containing an additional tandem repeat sequence (TRS), between and , with the exception of one strain from an ASFV-infected pig farm reported on 22 January 2023, which was an IGR I variant. In addition, a single-nucleotide polymorphism (SNP) was detected at position 107 from the start of the IGR between and in six isolates. The findings of this study suggest that the sources of the virus at the pig farms from which these variants originated differed from those of other pig farms.
在 2022 年至 2023 年 4 月暴发的疫情中，韩国有 15 个养猪场受到非洲猪瘟（ASF）的影响。从 15 个 ASFV 阳性养猪场的血液和组织样本中直接提取 ASF 病毒（ASFV）基因组，分析其遗传特征。系统进化分析显示，这 15 株病毒属于 p72 基因型 II 和 CD2v 血清群 8，是 基因中央可变区（CVR）I 的变异株。除了 2023 年 1 月 22 日报告的来自感染 ASF 猪的农场的 1 株病毒是 IGR I 变异株外，其余 14 株为 IGR II 变异株，在 和 之间存在额外的串联重复序列（TRS）。此外，在 6 个分离株中，在 和 之间 IGR 的 107 位处检测到单个核苷酸多态性（SNP）。本研究结果表明，这些变异株的病毒来源与其他养猪场不同。
