College of Agriculture and Life Sciences, Kyungpook National University, Daegu, 41566, Republic of Korea.
Institute of Plant Medicine, Kyungpook National University, Daegu, 41566, Republic of Korea.
Curr Microbiol. 2023 Aug 1;80(9):308. doi: 10.1007/s00284-023-03419-5.
A single Pectobacterium-like strain named 13-115 was isolated from a specimen of diseased cucumber stem tissue collected on Jeju Island, South Korea. The strain presented a rod-like shape and was negative for Gram staining. When grown on R2A medium at 25 °C, strain 13-115 formed round, convex and white colonies. This strain showed growth at temperatures ranging from 10 to 30 °C and tolerated a pH range of 6-9. The strain could also tolerate NaCl concentrations up to 5%. Analysis of the 16S rRNA gene sequence revealed that strain 13-115 exhibited similarity of over 99% with Pectobacterium brasiliense, P. carotovorum, P. polaris, and P. parvum. By conducting multilocus sequence analyses using dnaX, leuS, and recA genes, a separate phylogenetic lineage was discovered between strain 13-115 and other members of the genus Pectobacterium. Moreover, the strain showed relatively low in silico DNA-DNA hybridization (<60.6%) and average nucleotide identity (ANI) (<94.9%) values with recognized Pectobacterium species. The isolate has a genome size of 5,069,478 bp and a genomic G + C content of 52.04 mol%. Major fatty acids identified in the strain included C (28.99%), summed feature 3 (C ω7c and/or C ω6c; 28.85%), and C ω7c (19.01%). Pathogenicity assay confirmed that the novel strain induced soft rot symptoms in cucumber plants and Koch's postulates were fulfilled. Molecular analysis and phenotypic data indicated that strain 13-115 could be classified as a new species within the Pectobacterium genus, which has been named Pectobacterium jejuense. The type strain is 13-115 (= KCTC 92800 = JCM 35940).
从韩国济州岛采集的患病黄瓜茎组织标本中分离得到一株单一的类果胶杆菌菌株，命名为 13-115。该菌株呈杆状，革兰氏染色阴性。在 25°C 的 R2A 培养基上培养时，菌株 13-115 形成圆形、凸起和白色的菌落。该菌株在 10 至 30°C 的温度范围内生长，并能耐受 pH 值在 6-9 之间的范围。该菌株还能耐受高达 5%的 NaCl 浓度。16S rRNA 基因序列分析表明，菌株 13-115 与巴西果胶杆菌、胡萝卜软腐果胶杆菌、极地果胶杆菌和小菜蛾果胶杆菌的相似度超过 99%。通过对 dnaX、leuS 和 recA 基因进行多位点序列分析，发现菌株 13-115 与果胶杆菌属的其他成员之间存在一个单独的系统发育谱系。此外，该菌株与公认的果胶杆菌种的 DNA-DNA 杂交（<60.6%）和平均核苷酸同一性（ANI）（<94.9%）值相对较低。该分离株的基因组大小为 5069478bp，基因组 G+C 含量为 52.04mol%。该菌株中鉴定出的主要脂肪酸包括 C（28.99%）、总和特征 3（C ω7c 和/或 C ω6c；28.85%）和 C ω7c（19.01%）。致病性测定证实，该新型菌株能诱导黄瓜植株产生软腐病症状，柯赫氏假设得到满足。分子分析和表型数据表明，菌株 13-115 可归类为果胶杆菌属中的一个新种，命名为济州岛果胶杆菌。其模式株为 13-115（=KCTC 92800=JCM 35940）。
