Singh Priyanka, Katkar Pranav K, Walski Tomasz, Bohara Raghvendra A
Centre for Interdisciplinary Research, D.Y. Patil Educational Society, Kolhapur, India.
Department of Physics, Sejong University, Seoul, South Korea.
Heliyon. 2023 Jul 7;9(8):e18034. doi: 10.1016/j.heliyon.2023.e18034. eCollection 2023 Aug.
Yolk-shell-based silica-coated silver nanoparticles are prominently used in the biomedical field aas well as bare silver nanoparticles for various biological applications. The present work narrates the synthesis and silica coating of metallic silver nanoparticles and investigates their antibacterial, antifungal, and anticancerous activity. Both synthesized nanoparticles were characterized by TEM, and SEM-EDX. The average size of silver nanoparticles was 50 nm, while after coating with silica, the average size of silica-coated silver nanoparticles was 80 nm. The nanoparticles' antibacterial, antifungal, and anticancer properties were comparatively examined . Agar well diffusion method was employed to explore the antibacterial activity against gram-positive bacteria () and gram-negative bacteria () at different concentrations and antifungal activity against . To understand the minimum concentration of both nanoparticles, we employed the minimum inhibitory concentration (MIC) test, against bacterial and fungal strains, which was dose dependent. We learned that bare silver nanoparticles showed high antibacterial activity, whereas silica-coated silver nanoparticles surpassed their antifungal capability over bare silver nanoparticles against . The anticancer activity of the as-prepared nanoparticles was executed in opposition to the prostate cancer cell (PC-3) line by MTT assay, which showed meaningful activity. Following this, flow cytometry was also effectuated to learn about the number of apoptotic and necrotic cells. The results of this study demonstrate the dynamic anti-cancerous, antibacterial, and antifungal activities of bare silver nanoparticles and silica-coated silver nanoparticles for a long-lasting period.
基于蛋黄壳结构的二氧化硅包覆银纳米颗粒以及裸银纳米颗粒在各种生物医学应用中都有显著应用。本工作讲述了金属银纳米颗粒的合成及二氧化硅包覆过程，并研究了它们的抗菌、抗真菌和抗癌活性。两种合成的纳米颗粒均通过透射电子显微镜（TEM）、扫描电子显微镜-能谱仪（SEM-EDX）进行了表征。银纳米颗粒的平均尺寸为50纳米，而包覆二氧化硅后，二氧化硅包覆银纳米颗粒的平均尺寸为80纳米。对纳米颗粒的抗菌、抗真菌和抗癌性能进行了比较研究。采用琼脂孔扩散法探究在不同浓度下对革兰氏阳性菌（ ）和革兰氏阴性菌（ ）的抗菌活性以及对 的抗真菌活性。为了解两种纳米颗粒的最低浓度，我们针对细菌和真菌菌株进行了最低抑菌浓度（MIC）测试，该测试具有剂量依赖性。我们发现裸银纳米颗粒表现出较高的抗菌活性，而二氧化硅包覆银纳米颗粒在抗真菌能力方面超过了裸银纳米颗粒对 的效果。通过MTT法对所制备的纳米颗粒针对前列腺癌细胞（PC-3）系进行抗癌活性测试，结果显示出显著活性。在此之后，还进行了流式细胞术以了解凋亡和坏死细胞的数量。本研究结果表明裸银纳米颗粒和二氧化硅包覆银纳米颗粒在长期内具有动态的抗癌、抗菌和抗真菌活性。
