Sanders S J, Gavan T L, Senturia J B, Smeby R R
J Clin Microbiol. 1986 Sep;24(3):435-9. doi: 10.1128/jcm.24.3.435-439.1986.
Current methodology for the serum bactericidal test requires a minimum of 48 h. A procedure was devised for performing this test with the Autobac system (General Diagnostics, Div. Organon Inc., Raleigh, N.C.) in a shortened time span. All titers obtained with the Autobac were compared against results obtained with a standardized tube dilution procedure. The Autobac low-thymidine eugonic broth performed comparably to the tube dilution diluent, a 1:1 ratio of pooled human serum and cation-supplemented Mueller-Hinton broth (99.2% correlation between bactericidal endpoints). Over 300 tests were conducted by using stock reference bacterial strains, clinical isolates, pooled human serum seeded with antimicrobial agents, and serum from patients on antimicrobial therapy. With the Autobac procedure, serum inhibitory titers can be reported in 3 to 4 h (93.4% correlation with the tube dilution procedure). Serum bactericidal titers can be obtained in 24 h without the necessity of subculturing (95.6% correlation). With the exception of staphylococci tested against penicillin, serum bactericidal titers can be obtained in 3 to 4 h (88.4% correlation). The Autobac procedure can provide the clinical laboratory with a rapid, reliable method for performing the serum bactericidal test.
目前血清杀菌试验的方法至少需要48小时。我们设计了一种程序，可使用自动细菌鉴定系统（通用诊断公司，Organon公司分部，北卡罗来纳州罗利市）在较短时间内进行该试验。将自动细菌鉴定系统获得的所有效价与通过标准化试管稀释程序获得的结果进行比较。自动细菌鉴定系统的低胸腺嘧啶营养肉汤与试管稀释稀释剂表现相当，即混合人血清与补充阳离子的米勒-欣顿肉汤按1:1比例混合（杀菌终点之间的相关性为99.2%）。使用储备参考细菌菌株、临床分离株、接种抗菌剂的混合人血清以及接受抗菌治疗患者的血清进行了300多次试验。采用自动细菌鉴定系统程序，血清抑制效价可在3至4小时内报告（与试管稀释程序的相关性为93.4%）。无需传代培养，24小时即可获得血清杀菌效价（相关性为95.6%）。除了针对青霉素测试的葡萄球菌外，3至4小时内即可获得血清杀菌效价（相关性为88.4%）。自动细菌鉴定系统程序可为临床实验室提供一种快速、可靠的血清杀菌试验方法。
