Caparrós Esther, Cenit María Carmen, Muriel Javier, Benítez-Páez Alfonso, Moreno María Victoria, González-Delgado Purificación, Rubio Gonzalo, Sanz Yolanda, Fernández Javier
Clinical Medicine Department, University Miguel Hernández, San Juan de Alicante, Alicante, Spain.
Microbial Ecology, Nutrition, and Health Research Unit, Institute of Agrochemistry and Food Technology, Paterna, Valencia, Spain.
J Allergy Clin Immunol Glob. 2022 Sep 6;1(4):217-224. doi: 10.1016/j.jacig.2022.07.005. eCollection 2022 Nov.
Food protein-induced enterocolitis syndrome (FPIES) is a non-IgE-mediated food hypersensitivity that affects the gastrointestinal system, especially in children, who often present with more severe clinical manifestations than adults do. Although its pathogenesis is poorly understood and biomarkers are still lacking, scientific evidence suggests that gut microbiota may play an important role in the development of FPIES.
We aimed to compare the composition of gut microbiota in children with FPIES with that in age- and sex-matched healthy controls.
We analyzed the gut microbiota profiles in fecal samples of 17 patients with FPIES (case patients) and 12 age-matched healthy children (controls) by tag sequencing of the 16S ribosomal RNA gene hypervariable V4-V5 regions. Subjects' sociodemographic, clinical, and food diary variables were described and compared between groups by using inferential statistical tests. Nonparametric linear discriminant analysis was performed for intestinal microbiota data.
Patients with confirmed cases FPIES (n = 17; average patient age, 7.5 ± 3.2 years) and controls without FPIES or any atopy (n = 12, average patient age, 6.9 ± 2.7 years) were included. Fish was the main FPIES-inducing allergen in 65% of the cases. The patients with FPIES showed higher proportions of Lachnospiraceae spp ( < .0286) and a lower proportion of Ruminococcaceae spp ( < .0066), Lactobacillaceae spp ( < .0075), and Leuconostocaceae spp ( < .0173) than the controls.
Our data clearly show a different gut microbial signature in patients with FPIES, suggesting a new potential avenue for aiding the diagnosis and clinical management of FPIES. Larger studies are needed to confirm these results.
食物蛋白诱导的小肠结肠炎综合征（FPIES）是一种非IgE介导的食物过敏反应，影响胃肠道系统，尤其是儿童，他们通常比成人表现出更严重的临床表现。尽管其发病机制尚不清楚且仍缺乏生物标志物，但科学证据表明肠道微生物群可能在FPIES的发展中起重要作用。
我们旨在比较FPIES患儿与年龄和性别匹配的健康对照者的肠道微生物群组成。
我们通过对16S核糖体RNA基因高变V4-V5区域进行标签测序，分析了17例FPIES患者（病例组）和12例年龄匹配的健康儿童（对照组）粪便样本中的肠道微生物群谱。使用推断性统计检验描述并比较了两组受试者的社会人口统计学、临床和食物日记变量。对肠道微生物群数据进行非参数线性判别分析。
纳入确诊为FPIES的患者（n = 17；平均患者年龄，7.5 ± 3.2岁）和无FPIES或任何特应性疾病的对照组（n = 12，平均患者年龄，6.9 ± 2.7岁）。65%的病例中，鱼类是主要的FPIES诱导性过敏原。与对照组相比，FPIES患者的毛螺菌科菌属比例更高（P < .0286），而瘤胃球菌科菌属、乳杆菌科菌属和明串珠菌科菌属的比例更低（P < .0066、P < .0075、P < .0173）。
我们的数据清楚地显示了FPIES患者不同的肠道微生物特征，这为辅助FPIES的诊断和临床管理提供了一条新的潜在途径。需要更大规模的研究来证实这些结果。
