Faculty of Health Sciences, Ben-Gurion University of the Negev, The International Center of Health, Innovation & Nutrition On the memory of Manya Igel, Beer-Sheva, Israel.
Department of Medicine, Hebrew University and Hadassah Medical Center, Jerusalem, Israel.
Gut Microbes. 2023 Dec;15(2):2264457. doi: 10.1080/19490976.2023.2264457. Epub 2023 Oct 5.
We previously reported that autologous-fecal-microbiota-transplantation (aFMT), following 6 m of lifestyle intervention, attenuated subsequent weight regain and insulin rebound for participants consuming a high-polyphenol green-Mediterranean diet. Here, we explored whether specific changes in the core (abundant) vs. non-core (low-abundance) gut microbiome taxa fractions during the weight-loss phase (0-6 m) were differentially associated with weight maintenance following aFMT. Eighty-two abdominally obese/dyslipidemic participants (age = 52 years; 6 m weightloss = -8.3 kg) who provided fecal samples (0 m, 6 m) were included. Frozen 6 m's fecal samples were processed into 1 g, opaque and odorless aFMT capsules. Participants were randomly assigned to receive 100 capsules containing their own fecal microbiota or placebo over 8 m-14 m in ten administrations (adherence rate > 90%). Gut microbiome composition was evaluated using shotgun metagenomic sequencing. Non-core taxa were defined as ≤ 66% prevalence across participants. Overall, 450 species were analyzed. At baseline, 13.3% were classified as core, and Firmicutes presented the highest core proportion by phylum. During 6 m weight-loss phase, abundance of non-core species changed more than core species ( < .0001). Subject-specific changes in core and non-core taxa fractions were strongly correlated (Jaccard Index; = 0.54; < .001). Following aFMT treatment, only participants with a low 6 m change in core taxa, and a high change in non-core taxa, avoided 8-14 m weight regain (aFMT = -0.58 ± 2.4 kg, corresponding placebo group = 3.18 ± 3.5 kg; = .02). In a linear regression model, 6 m change was the only combination that was significantly associated with attenuated 8-14 m weight regain ( = .038; = .002 for taxa patterns/treatment intervention interaction). High change in non-core, low-abundance taxa during weight-loss might mediate aFMT treatment success for weight loss maintenance. NCT03020186.
我们之前曾报道，在进行 6 个月的生活方式干预后，自体粪菌移植（aFMT）可减轻参与者在摄入高多酚的绿色地中海饮食后体重的反弹和胰岛素的反弹。在这里，我们探讨了在减重阶段（0-6 个月）核心（丰富）与非核心（低丰度）肠道微生物群分类群分数的特定变化是否与 aFMT 后体重维持有差异。82 名腹部肥胖/血脂异常的参与者（年龄=52 岁；6 个月体重减轻= -8.3 公斤）提供了粪便样本（0 个月，6 个月）。将冷冻的 6 个月粪便样本加工成 1 克、不透明且无味的 aFMT 胶囊。参与者被随机分配在 8-14 个月内通过 10 次给药（依从率> 90%）接受 100 粒含有自身粪便微生物群的胶囊或安慰剂。使用 shotgun 宏基因组测序评估肠道微生物组组成。非核心分类群定义为在所有参与者中患病率低于 66%。总体而言，分析了 450 个物种。在基线时，有 13.3%被归类为核心，厚壁菌门的核心比例最高。在 6 个月的减肥阶段，非核心物种的丰度变化大于核心物种（ < .0001）。个体特异性核心和非核心分类群分数的变化呈强相关（Jaccard 指数； = 0.54； < .001）。接受 aFMT 治疗后，只有那些在 6 个月时核心分类群变化较低，而非核心分类群变化较高的参与者，才能避免 8-14 个月的体重反弹（aFMT= -0.58 ± 2.4 公斤，相应的安慰剂组= 3.18 ± 3.5 公斤； = .02）。在一个线性回归模型中，6 个月的变化是唯一与减轻 8-14 个月体重反弹显著相关的组合（ = .038； = .002 用于分类群模式/治疗干预相互作用）。在减肥期间，非核心的高丰度、低丰度分类群的变化可能介导了 aFMT 治疗对体重维持的成功。NCT03020186。
