Peng Ruolin, Zhang Zhenyu, Qu Yi, Chen Weiwei
Department of Gastroenterology, Nanjing First Hospital, Nanjing Medical University, Nanjing, China.
Front Microbiol. 2023 Oct 2;14:1273709. doi: 10.3389/fmicb.2023.1273709. eCollection 2023.
infection and eradication have been reported to cause dysbiosis of the oral microbiota. Probiotics are increasingly being used to maintain the balance of the oral microbiota. We aimed to investigate the effects of infection, eradication with vonoprazan-amoxicillin dual therapy, and probiotics supplementation on the oral microbiota.
positive patients were randomly assigned to a vonoprazan-amoxicillin regimen plus probiotics (BtT group) or the placebo (PT group) for 14 days. negative population served as normal controls. Tongue coating samples were collected from 60 positive patients at three time points (before eradication, after eradication, and at confirmation of infection cure) and 20 negative subjects. 16S rRNA gene sequencing was used to analyze the oral microbiota.
was detected in the oral cavity in positive (34/60), negative (7/20), and eradicated (1/60) subjects using high-throughput sequencing. Compared with normal controls, positive patients exhibited higher richness ( = 0.012) and comparable diversity ( = 0.075) of oral microbiota. Beta diversity and KEGG analysis showed oral flora composition and function differences in positive and negative subjects. Alpha diversity dramatically decreased after eradication and modestly increased with confirmation of eradication. Beta diversity and LEfSe analysis revealed distinct structures, and KEGG analysis showed distinct signaling pathways of tongue coating flora at three time points. There was a significant reduction of Firmicutes and after erdication. The PT group and BtT group had identical compositional and functional differences of oral microbiota at three time points.
No substantial link existed between oral and stomach , while removing gastric helped eliminate oral . infection and vonoprazan-amoxicillin dual therapy affected oral microbiota diversity, structure, and function. eradication demonstrated a suppressive impact on the proliferation of oral pathogens, specifically Firmicutes and . Nevertheless, probiotics supplementation did not reduce the oral microbial disturbance caused by eradication.
https://www.chictr.org.cn/, identifiers CHICTR2200060023.
据报道，幽门螺杆菌感染及根除会导致口腔微生物群失调。益生菌越来越多地被用于维持口腔微生物群的平衡。我们旨在研究幽门螺杆菌感染、沃克帕唑-阿莫西林联合疗法根除幽门螺杆菌以及补充益生菌对口腔微生物群的影响。
幽门螺杆菌阳性患者被随机分为沃克帕唑-阿莫西林方案加益生菌组（BtT组）或安慰剂组（PT组），治疗14天。幽门螺杆菌阴性人群作为正常对照。在三个时间点（根除前、根除后以及确认幽门螺杆菌感染治愈时）从60例幽门螺杆菌阳性患者和20例阴性受试者中采集舌苔样本。采用16S rRNA基因测序分析口腔微生物群。
通过高通量测序在幽门螺杆菌阳性（34/60）、阴性（7/20）和根除（1/60）受试者的口腔中检测到幽门螺杆菌。与正常对照相比，幽门螺杆菌阳性患者口腔微生物群的丰富度更高（P = 0.012），多样性相当（P = 0.075）。β多样性和KEGG分析显示幽门螺杆菌阳性和阴性受试者的口腔菌群组成和功能存在差异。根除幽门螺杆菌后α多样性显著降低，确认根除后略有增加。β多样性和LEfSe分析揭示了三个时间点舌苔菌群的不同结构，KEGG分析显示了不同的信号通路。根除幽门螺杆菌后厚壁菌门和幽门螺杆菌显著减少。PT组和BtT组在三个时间点口腔微生物群的组成和功能差异相同。
口腔和胃中的幽门螺杆菌之间不存在实质性联系，而清除胃内幽门螺杆菌有助于消除口腔中的幽门螺杆菌。幽门螺杆菌感染和沃克帕唑-阿莫西林联合疗法影响口腔微生物群的多样性、结构和功能。根除幽门螺杆菌对口腔病原体，特别是厚壁菌门和幽门螺杆菌的增殖有抑制作用。然而，补充益生菌并不能减少根除幽门螺杆菌引起的口腔微生物紊乱。
