Fuentes Yihajara, Giovagnoli-Vicuña Claudia, Faúndez Mario, Giordano Ady
Departamento de Química Inorgánica, Escuela de Química, Facultad de Química y de Farmacia, Pontificia Universidad Católica de Chile, Macul 7820436, Chile.
Departamento de Farmacia, Escuela de Química y Farmacia, Facultad de Química y de Farmacia, Pontificia Universidad Católica de Chile, Macul 7820436, Chile.
Antioxidants (Basel). 2023 Oct 23;12(10):1900. doi: 10.3390/antiox12101900.
The microencapsulation of bioactive extracts of Chilean papaya waste, including both seeds and skin, was investigated. Papaya waste extract microcapsules utilizing maltodextrin at 10% (MD10), 20% (MD20), and 30% (MD30) (/) as the wall material through the freeze-drying process were obtained, and subsequently their physicochemical, antioxidant, and antimicrobial properties were evaluated. The TPC efficiency and yield values achieved were more than 60% for the microencapsulated seed and skin extracts, respectively. The best results for phenolic and antioxidant compounds were found in the microencapsulated seed extract with MD20, with a value of 44.20 ± 3.32 EAG/g DW for total phenols and an antioxidant capacity of 12.0 ± 0.32 mol ET/g DW for the DPPH and 236.3 ± 4.1 mol ET/g DW for the FRAP assay. In addition, the seed and skin samples reduced ROS generation in HO-treated Hek293 cells. In terms of antimicrobial activity, values ranging from 7 to 15 mm of inhibitory halos were found, with the maximum value corresponding to the inhibition of , for both microencapsulated extracts. Therefore, the successful microencapsulation of the waste bioactive extracts (seed and skin) with the demonstrated antimicrobial and antioxidant properties highlight the bioactivity from Chilean papaya waste resources.
对智利木瓜废弃物（包括种子和果皮）的生物活性提取物进行了微胶囊化研究。通过冷冻干燥工艺，以10%（MD10）、20%（MD20）和30%（MD30）的麦芽糊精作为壁材，制备了木瓜废弃物提取物微胶囊，随后对其物理化学性质、抗氧化性能和抗菌性能进行了评估。微胶囊化的种子提取物和果皮提取物的总酚含量效率和产率分别超过60%。在以MD20制备的微胶囊化种子提取物中，酚类和抗氧化化合物的含量最高，总酚含量为44.20±3.32 EAG/g干重，DPPH法测定的抗氧化能力为12.0±0.32 mol ET/g干重，FRAP法测定的抗氧化能力为236.3±4.1 mol ET/g干重。此外，种子和果皮样品减少了过氧化氢处理的Hek293细胞中活性氧的产生。在抗菌活性方面，两种微胶囊化提取物的抑菌圈直径在7至15毫米之间，最大值对应于对[此处原文缺失具体微生物名称]的抑制。因此，成功地将具有抗菌和抗氧化性能的废弃物生物活性提取物（种子和果皮）微胶囊化，突出了智利木瓜废弃物资源的生物活性。
