Chen Jonathan H K, Leung Hoi-Yi, Wong Charles M C, Yuen Kwok-Yung, Cheng Vincent C C
Department of Microbiology, Queen Mary Hospital, Hong Kong SAR, China.
Department of Microbiology, School of Clinical Medicine, The University of Hong Kong, Hong Kong SAR, China.
Microorganisms. 2023 Sep 28;11(10):2435. doi: 10.3390/microorganisms11102435.
is a novel Staphylococcus species derived from . Information on the prevalence and genetic characteristics of invasive in Asia is limited. In this study, 275 invasive complex strains were retrieved from blood culture specimens in Hong Kong and re-analyzed using MALDI-TOF mass spectrometry and an in-house multiplex real-time PCR for . The prevalence of invasive in Hong Kong was found to be 4.0% (11/275). These strains were primarily susceptible to commonly used antibiotics, except penicillin. Whole-genome sequencing revealed the circulation of three genotypes (ST-2250, ST-1223, and ST-2854) in Hong Kong, with ST-2250 and ST-1223 being the predominant genotypes. The local ST-2250 and ST-1223 strains showed close phylogenetic relationships with isolates from mainland China. Antimicrobial-resistant genes (, , , , ) could be found in nearly all local strains. The ST-1223 and ST-2250 genotypes carried multiple staphylococcal enterotoxin genes that could cause food poisoning and toxic shock syndrome. The CRISPR/Cas locus was observed only in the ST-2250 strains. This study provides the first report on the molecular epidemiology of invasive in Hong Kong, and further analysis is needed to understand its transmission reservoir.
是一种源自……的新型葡萄球菌物种。关于亚洲侵袭性……的流行情况和基因特征的信息有限。在本研究中，从香港的血培养标本中检索出275株侵袭性……复合菌株，并使用基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）和内部多重实时聚合酶链反应（PCR）对……进行重新分析。发现香港侵袭性……的流行率为4.0%（11/275）。这些菌株除对青霉素外，主要对常用抗生素敏感。全基因组测序揭示了香港三种……基因型（ST-2250、ST-1223和ST-2854）的传播情况，其中ST-2250和ST-1223是主要基因型。本地的ST-2250和ST-1223菌株与来自中国大陆的分离株显示出密切的系统发育关系。几乎所有本地……菌株中都可发现抗微生物耐药基因（……）。ST-1223和ST-2250基因型携带多个可导致食物中毒和中毒性休克综合征的葡萄球菌肠毒素基因。CRISPR/Cas位点仅在ST-2250菌株中观察到。本研究首次报告了香港侵袭性……的分子流行病学情况，需要进一步分析以了解其传播宿主。
