Jiang Haihong, Li Xueyi, Chen Tianhong, Liu Yang, Wang Qian, Wang Zhimin, Jia Jia
School of Life Sciences, Shanghai University, Shanghai, China.
Sino-Swiss Institute of Advanced Technology, School of Micro-electronics, Shanghai University, Shanghai, China.
Mater Today Bio. 2023 Oct 28;23:100846. doi: 10.1016/j.mtbio.2023.100846. eCollection 2023 Dec.
3D bioprinting technology is widely used to fabricate various tissue structures. However, the absence of vessels hampers the ability of bioprinted tissues to receive oxygen and nutrients as well as to remove wastes, leading to a significant reduction in their survival rate. Despite the advancements in bioinks and bioprinting technologies, bioprinted vascular structures continue to be unsuitable for transplantation compared to natural blood vessels. In addition, a complete assessment index system for evaluating the structure and function of bioprinted vessels in vitro has not yet been established. Therefore, in this review, we firstly highlight the significance of selecting suitable bioinks and bioprinting techniques as they two synergize with each other. Subsequently, focusing on both vascular-associated cells and vascular tissues, we provide a relatively thorough assessment of the functions of bioprinted vascular tissue based on the physiological functions that natural blood vessels possess. We end with a review of the applications of vascular models, such as vessel-on-a-chip, in simulating pathological processes and conducting drug screening at the organ level. We believe that the development of fully functional blood vessels will soon make great contributions to tissue engineering and regenerative medicine.
3D生物打印技术被广泛用于制造各种组织结构。然而，血管的缺失阻碍了生物打印组织获取氧气和营养物质以及排出废物的能力，导致其存活率显著降低。尽管生物墨水和生物打印技术取得了进展，但与天然血管相比，生物打印的血管结构仍然不适合移植。此外，尚未建立用于体外评估生物打印血管结构和功能的完整评估指标体系。因此，在本综述中，我们首先强调选择合适的生物墨水和生物打印技术的重要性，因为它们两者相互协同作用。随后，聚焦于血管相关细胞和血管组织，我们基于天然血管所具有的生理功能，对生物打印血管组织的功能进行了较为全面的评估。我们最后回顾了血管模型（如芯片上的血管）在模拟病理过程和进行器官水平药物筛选方面的应用。我们相信，功能完备的血管的发展将很快为组织工程和再生医学做出巨大贡献。
