2nd Department of Radiology, Medical School, Attikon University Hospital, National and Kapodistrian University of Athens, 11527 Athens, Greece.
Medical Physics Program, Department of Physics and Applied Physics, Kennedy College of Sciences, University of Massachusetts Lowell, 265 Riverside St., Lowell, MA 01854, USA.
Biomolecules. 2023 Nov 29;13(12):1720. doi: 10.3390/biom13121720.
Cervical cancer remains a pressing global health concern, necessitating advanced therapeutic strategies. Radiotherapy, a fundamental treatment modality, has faced challenges such as targeted dose deposition and radiation exposure to healthy tissues, limiting optimal outcomes. To address these hurdles, nanomaterials, specifically gold nanoparticles (AuNPs), have emerged as a promising avenue. This study delves into the realm of cervical cancer radiotherapy through the meticulous exploration of AuNPs' impact. Utilizing ex vivo experiments involving cell lines, this research dissected intricate radiobiological interactions. Detailed scrutiny of cell survival curves, dose enhancement factors (DEFs), and apoptosis in both cancer and normal cervical cells revealed profound insights. The outcomes showcased the substantial enhancement of radiation responses in cancer cells following AuNP treatment, resulting in heightened cell death and apoptotic levels. Significantly, the most pronounced effects were observed 24 h post-irradiation, emphasizing the pivotal role of timing in AuNPs' efficacy. Importantly, AuNPs exhibited targeted precision, selectively impacting cancer cells while preserving normal cells. This study illuminates the potential of AuNPs as potent radiosensitizers in cervical cancer therapy, offering a tailored and efficient approach. Through meticulous ex vivo experimentation, this research expands our comprehension of the complex dynamics between AuNPs and cells, laying the foundation for their optimized clinical utilization.
宫颈癌仍然是一个紧迫的全球健康问题，需要先进的治疗策略。放疗是一种基本的治疗方式，但它面临着靶向剂量沉积和对健康组织的辐射暴露等挑战，限制了最佳治疗效果。为了解决这些难题，纳米材料，特别是金纳米颗粒（AuNPs），已经成为一个很有前途的途径。本研究通过细致探索 AuNPs 对宫颈癌放疗的影响，深入研究了宫颈癌放疗。本研究利用涉及细胞系的离体实验，剖析了复杂的放射生物学相互作用。详细研究了癌细胞和正常宫颈细胞的细胞存活曲线、剂量增强因子（DEF）和细胞凋亡，揭示了深刻的见解。结果显示，AuNP 处理后癌细胞对辐射的反应显著增强，导致细胞死亡和凋亡水平升高。重要的是，在辐照后 24 小时观察到最显著的效果，这强调了 AuNPs 疗效中时间的关键作用。重要的是，AuNPs 表现出靶向精度，选择性地影响癌细胞，同时保护正常细胞。本研究揭示了 AuNPs 作为宫颈癌治疗中有效的放射增敏剂的潜力，提供了一种针对性强、效率高的方法。通过细致的离体实验，本研究扩展了我们对 AuNPs 和细胞之间复杂动态的理解，为它们的临床优化应用奠定了基础。
