Yang Ru, Wang Yinan, Ying Zhiye, Shi Zeyao, Song Yan, Yan Jing, Hou Shulin, Zhao Zicheng, Hu Yanling, Chen Qiong, Peng Wentao, Li Xiaowen
Department of Neonatology Nursing, West China Second University Hospital, Sichuan University, Chengdu, China.
Key Laboratory of Birth Defects and Related Diseases of Women and Children, Sichuan University, Ministry of Education, Chengdu, China.
Front Microbiol. 2024 Feb 29;15:1292377. doi: 10.3389/fmicb.2024.1292377. eCollection 2024.
The initial acquisition and subsequent development of the microbiota in early life is crucial to future health. Cesarean-section (CS) birth is considered to affect early microbial transmission from mother to infant.
In this study, we collected fecal samples from 34 CS infants and their mothers from West China Second Hospital, Sichuan University to assess the microbiota developmental trajectory of mothers and infants. We explored mother-infant gut microbiome transmission via comparison with corresponding Finnish data.
Metagenomic analysis of gut microbiota profiles indicated that the communities of mothers and infants were distinct. The composition of the infant gut microbiome was highly variable but also followed predictable patterns in the early stages of life. Maternal communities were stable and mainly dominated by species from spp. We used PStrain to analyze and visualize strain transmission in each mother-infant pair. Excluding missing data, we included 32 mother-infant pairs for analysis of strain transmission. Most CS deliveries (65.6%, 21/32) did not demonstrate transmission of strains from mother to infant. To further explore the mother-infant strain transmission, we analyzed metagenomics data from Finnish mother-infant pairs. A total of 32 mother-infant pairs were included in the analysis, including 28 vaginal delivery (VD) infants and four CS infants. Strain transmission was observed in 30 infants, including 28 VD infants and two CS infants. All VD infants received transmitted stains from their mothers. Finally, a total of 193 strain transmission events were observed, comprising 131 strains and 45 species.
Taken together, our data suggested that delivery mode was an important factor influencing the mother-infant strain transmission.
生命早期微生物群的初始获得及其后续发展对未来健康至关重要。剖宫产出生被认为会影响母婴之间早期的微生物传播。
在本研究中，我们从四川大学华西第二医院收集了34对剖宫产母婴的粪便样本，以评估母婴的微生物群发育轨迹。通过与相应的芬兰数据进行比较，我们探索了母婴肠道微生物组的传播情况。
肠道微生物群概况的宏基因组分析表明，母婴群落不同。婴儿肠道微生物组的组成高度可变，但在生命早期也遵循可预测的模式。母亲的群落稳定，主要由来自……属的物种主导。我们使用PStrain分析并可视化每对母婴中的菌株传播情况。排除缺失数据后，我们纳入了32对母婴进行菌株传播分析。大多数剖宫产分娩（65.6%，21/32）未显示出菌株从母亲传播给婴儿。为了进一步探索母婴菌株传播情况，我们分析了来自芬兰母婴对的宏基因组数据。分析共纳入32对母婴，包括28例阴道分娩（VD）婴儿和4例剖宫产婴儿。在30例婴儿中观察到菌株传播，包括28例VD婴儿和2例剖宫产婴儿。所有VD婴儿都从母亲那里获得了传播的菌株。最后，共观察到193次菌株传播事件，包括131株菌株和45个物种。
综上所述，我们的数据表明分娩方式是影响母婴菌株传播的一个重要因素。
