Zhou Hui, Li Wenwei, Pan Lixin, Zhu Tianci, Zhou Teng, Xiao E, Wei Qiang
State Key Laboratory of Polymer Materials and Engineering, College of Polymer Science and Engineering, Sichuan University, Chengdu 610065, China.
Hunan Maybio Bio-Pharmaceutical Co., Ltd, Changsha 410000, China.
Regen Biomater. 2024 Feb 1;11:rbae008. doi: 10.1093/rb/rbae008. eCollection 2024.
Collagen, the most abundant structural protein in the human extracellular matrix (ECM), provides essential support for tissues and guides tissue development. Despite its widespread use in tissue engineering, there remains uncertainty regarding the optimal selection of collagen sources. Animal-derived sources pose challenges such as immunogenicity, while the recombinant system is hindered by diminished bioactivity. In this study, we hypothesized that human ECM-like collagen (hCol) could offer an alternative for tissue engineering. In this study, a facile platform was provided for generating hCol derived from mesenchymal stem cells with a hierarchical structure and biochemical properties resembling native collagen. Our results further demonstrated that hCol could facilitate basal biological behaviors of human adipose-derived stem cells, including viability, proliferation, migration and adipocyte-like phenotype. Additionally, it could promote cutaneous wound closure. Due to its high similarity to native collagen and good bioactivity, hCol holds promise as a prospective candidate for and applications in tissue engineering.
胶原蛋白是人体细胞外基质（ECM）中含量最丰富的结构蛋白，为组织提供重要支撑并引导组织发育。尽管其在组织工程中得到广泛应用，但关于胶原蛋白来源的最佳选择仍存在不确定性。动物来源的胶原蛋白存在免疫原性等挑战，而重组系统则受到生物活性降低的阻碍。在本研究中，我们假设人ECM样胶原蛋白（hCol）可为组织工程提供一种替代方案。在本研究中，提供了一个简便的平台，用于生成源自间充质干细胞的具有分层结构和类似于天然胶原蛋白生化特性的hCol。我们的结果进一步表明，hCol可促进人脂肪来源干细胞的基础生物学行为，包括活力、增殖、迁移和脂肪细胞样表型。此外，它还可促进皮肤伤口愈合。由于其与天然胶原蛋白高度相似且具有良好的生物活性，hCol有望成为组织工程中一种有前景的候选材料并应用于相关领域。
