Mond J J, Thompson C, Finkelman F D, Farrar J, Schaefer M, Robb R J
Proc Natl Acad Sci U S A. 1985 Mar;82(5):1518-21. doi: 10.1073/pnas.82.5.1518.
Immunoaffinity-purified interleukin 2 (IL2) stimulated proliferation of large but not small B cells. Stimulation was observed even when B cells were cultured at very low cell densities (3 X 10(4) per microwell containing 0.2 ml of medium). Addition of small numbers of purified splenic T cells did not enhance the IL2-induced B-cell proliferative response. These results suggest that IL2 was not operating through contaminating T cells. B cells cultured with anti-Ig antibody in vitro showed enhanced proliferation when cultured with EL4 thymoma-derived B-cell growth factor but not when cultured with IL2. A direct role for IL2 in B-cell activation is discussed.
免疫亲和纯化的白细胞介素2（IL2）刺激大B细胞而非小B细胞增殖。即便B细胞在非常低的细胞密度（每含0.2毫升培养基的微孔中3×10⁴个细胞）下培养，仍可观察到刺激作用。添加少量纯化的脾T细胞并不能增强IL2诱导的B细胞增殖反应。这些结果表明IL2并非通过污染的T细胞发挥作用。体外与抗Ig抗体一起培养的B细胞，与EL4胸腺瘤衍生的B细胞生长因子一起培养时增殖增强，但与IL2一起培养时则不然。本文讨论了IL2在B细胞激活中的直接作用。
