亲和纯化的白细胞介素2可诱导大B细胞而非小B细胞增殖。
Affinity-purified interleukin 2 induces proliferation of large but not small B cells.
作者信息
Mond J J, Thompson C, Finkelman F D, Farrar J, Schaefer M, Robb R J
出版信息
Proc Natl Acad Sci U S A. 1985 Mar;82(5):1518-21. doi: 10.1073/pnas.82.5.1518.
Abstract
Immunoaffinity-purified interleukin 2 (IL2) stimulated proliferation of large but not small B cells. Stimulation was observed even when B cells were cultured at very low cell densities (3 X 10(4) per microwell containing 0.2 ml of medium). Addition of small numbers of purified splenic T cells did not enhance the IL2-induced B-cell proliferative response. These results suggest that IL2 was not operating through contaminating T cells. B cells cultured with anti-Ig antibody in vitro showed enhanced proliferation when cultured with EL4 thymoma-derived B-cell growth factor but not when cultured with IL2. A direct role for IL2 in B-cell activation is discussed.
摘要
免疫亲和纯化的白细胞介素2（IL2）刺激大B细胞而非小B细胞增殖。即便B细胞在非常低的细胞密度（每含0.2毫升培养基的微孔中3×10⁴个细胞）下培养，仍可观察到刺激作用。添加少量纯化的脾T细胞并不能增强IL2诱导的B细胞增殖反应。这些结果表明IL2并非通过污染的T细胞发挥作用。体外与抗Ig抗体一起培养的B细胞，与EL4胸腺瘤衍生的B细胞生长因子一起培养时增殖增强，但与IL2一起培养时则不然。本文讨论了IL2在B细胞激活中的直接作用。
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本文引用的文献
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Proliferation and differentiation of single hapten-specific B lymphocytes is promoted by T-cell factor(s) distinct from T-cell growth factor.不同于T细胞生长因子的T细胞因子可促进单个半抗原特异性B淋巴细胞的增殖和分化。
Proc Natl Acad Sci U S A. 1982 Oct;79(20):6350-4. doi: 10.1073/pnas.79.20.6350.
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