Pringle A T, Forsdyke J, Rose A H
J Bacteriol. 1979 Oct;140(1):289-93. doi: 10.1128/jb.140.1.289-293.1979.
A suspension of Saccharomyces cerevisiae NCY366 in buffered 1.2 M sorbitol containing Zymolyase-5000 (a beta-glucanase-containing preparation/showed maximum osmotic sensitivity after 30 min of incubation at 30 degrees C. A scanning electron microscope study of spheroplast formation, using a very high resolution (4-nm) machine, revealed several new morphological features. The surface of the plug in bud scars on intact cells appeared warty. The wall, which assumed a beady appearance as digestion proceded, ultimately sloughed off to reveal the furrowed surface of the plasma membrane. Bud scars were resistant to digestion and. as incubation proceeded, they became surrounded by an outer annulus, which may be the seconary septum. Wall material was completely removed from the majority of cells only after 60 min of digestion. The surface of spheroplasts was studded with particles, about 25 to 30 nm in diameter. Many spheroplasts had a single large indentation, which may be in that part of the plasma membrane originally underlying the birth scar.
酿酒酵母NCY366悬浮于含有溶壁酶-5000（一种含β-葡聚糖酶的制剂）的1.2M山梨醇缓冲液中，在30℃孵育30分钟后显示出最大的渗透敏感性。使用超高分辨率（4纳米）仪器对原生质球形成进行的扫描电子显微镜研究揭示了几个新的形态特征。完整细胞芽痕处的塞子表面看起来有疣状突起。随着消化过程的进行，细胞壁呈现出珠子状外观，最终脱落，露出有沟纹的质膜表面。芽痕对消化有抗性，随着孵育的进行，它们被一个外环包围，这个外环可能是次生隔膜。只有在消化60分钟后，大部分细胞的细胞壁物质才被完全去除。原生质球表面布满了直径约25至30纳米的颗粒。许多原生质球有一个大的凹陷，可能位于原来位于出生痕下方的质膜部分。
