流式细胞术在嗜肺军团菌检测与鉴定中的应用
Application of flow cytometry to detection and characterization of Legionella spp.
作者信息
Tyndall R L, Hand R E, Mann R C, Evans C, Jernigan R
出版信息
Appl Environ Microbiol. 1985 Apr;49(4):852-7. doi: 10.1128/aem.49.4.852-857.1985.
Abstract
Flow cytometry, using fluorescein-bound specific antibodies and propidium iodide, was shown to be effective in detecting Legionella spp. in cooling tower waters. The procedure was quicker and less labor intensive than fluorescent microscopy. The use of these procedures also identified qualitative differences, perhaps related to infectivity, in Legionella populations.
摘要
流式细胞术使用荧光素结合的特异性抗体和碘化丙啶，已证明在检测冷却塔水中的军团菌属方面是有效的。该程序比荧光显微镜检查更快且劳动强度更低。这些程序的使用还确定了军团菌群体中的定性差异，可能与感染性有关。
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本文引用的文献
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