Adamberg Signe, Rasmussen Torben Sølbeck, Larsen Sabina Brigitte, Mao Xiaotian, Nielsen Dennis Sandris, Adamberg Kaarel
Department of Chemistry and Biotechnology, Tallinn University of Technology, Akadeemia tee 15, Tallinn, Estonia.
Section of Food Microbiology, Gut Health, and Fermentation, Department of Food Science, University of Copenhagen, Rolighedsvej 26, Frederiksberg, Denmark.
iScience. 2024 Jul 5;27(8):110460. doi: 10.1016/j.isci.2024.110460. eCollection 2024 Aug 16.
Recent studies indicate an important role of bacteriophages for successful fecal microbiota transplantation (FMT). However, wider clinical applications of FMT are hampered by to donor variability and concerns of infection risks by bacteria and human viruses. To overcome these challenges, mouse cecal and human fecal material were propagated in a chemostat fermentation setup supporting multiplication of bacteria, and phages, while propagation of eukaryotic viruses will be prevented in the absence of eukaryotic host cells. The results showed decrease of the median relative abundance of viral contigs of classified eukaryotic viruses below 0.01%. The corresponding virome profiles showed dilution rate dependency, a reproducibility between biological replicates, and maintained high diversity regarding both the human and mouse inocula. This proof-of-concept cultivation approach may constitute the first step of developing novel therapeutic tools with high reproducibility and with low risk of infection from the donor material to target gut-related diseases.
最近的研究表明噬菌体在成功进行粪便微生物群移植（FMT）中起着重要作用。然而，FMT更广泛的临床应用受到供体变异性以及细菌和人类病毒感染风险担忧的阻碍。为了克服这些挑战，在支持细菌和噬菌体增殖的恒化器发酵装置中培养小鼠盲肠和人类粪便样本，而在没有真核宿主细胞的情况下可防止真核病毒的增殖。结果显示，分类的真核病毒的病毒重叠群的中位相对丰度降至0.01%以下。相应的病毒组图谱显示出稀释率依赖性、生物学重复之间的可重复性，并且在人类和小鼠接种物方面都保持了高度多样性。这种概念验证培养方法可能构成开发具有高可重复性且从供体材料到目标肠道相关疾病感染风险低的新型治疗工具的第一步。
