State Key Laboratory of Oral Diseases, National Center for Stomatology, and National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, P.R. China.
Department of Cardiology and Endodontics, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, P.R. China.
Sci Adv. 2024 Sep 20;10(38):eado0016. doi: 10.1126/sciadv.ado0016.
in colorectal cancer (CRC) tissue is implicated at multiple stages of the disease, while the mechanisms underlying bacterial translocation and colonization remain incompletely understood. Herein, we investigated whether extracellular vesicles derived from (FnEVs) have impacts on bacterial colonization. In mice with colitis-related CRC, a notable enrichment of FnEVs was observed, leading to a significant increase in intratumor colonization by and accelerated progression of CRC. The enrichment of FnEVs in clinical CRC tissues was demonstrated. Subsequently, we revealed that FnEVs undergo membrane fusion with CRC cells, leading to the transfer and retention of FomA on recipient cell surfaces. Given its ability to facilitate autoaggregation through interaction with FN1441, the presence of FomA on CRC cell surfaces presents a target for bacterial adhesion. Collectively, the findings unveil a mechanism used by EVs to prepare a niche conducive for bacterial colonization in distal organs.
在结直肠癌 (CRC) 组织中，它在疾病的多个阶段都有涉及，而细菌易位和定植的机制仍不完全清楚。在此，我们研究了源自 （FnEVs）的细胞外囊泡是否对细菌定植有影响。在结肠炎相关 CRC 的小鼠中，观察到 FnEVs 的明显富集，导致肿瘤内 定植显著增加，并加速 CRC 的进展。在临床 CRC 组织中证实了 FnEVs 的富集。随后，我们揭示了 FnEVs 与 CRC 细胞发生膜融合，导致 FomA 在受体细胞表面的转移和保留。由于其通过与 FN1441 相互作用促进 自动聚集的能力，CRC 细胞表面的 FomA 成为细菌黏附的靶点。总的来说，这些发现揭示了 EVs 用于在远端器官中为细菌定植准备适宜小生境的机制。
