Barthold Jeanne E, Cai Luyao, McCreery Kaitlin P, Fischenich Kristine M, Eckstein Kevin N, Ferguson Virginia L, Emery Nancy C, Breur Gert, Neu Corey P
Department of Mechanical Engineering, University of Colorado Boulder, Boulder, CO, USA.
Weldon School of Biomedical Engineering, Purdue University, West Lafayette, IN, USA.
NPJ Regen Med. 2024 Sep 28;9(1):25. doi: 10.1038/s41536-024-00367-x.
The repair of articular cartilage after damage is challenging, and decellularized tissue offers a possible treatment option to promote regeneration. Here, we show that acellular osteochondral allografts improve integrative cartilage repair compared to untreated defects after 6 months in an ovine model. Functional measures of intratissue strain/structure assessed by MRI demonstrate similar biomechanics of implants and native cartilage. Compared to native tissue and defects, the structure, composition, and tribology of acellular allografts preserve surface roughness and lubrication, material properties under compression and relaxation, compositional ratios of collagen:glycosaminoglycan and collagen:phosphate, and relative composition of types I/II collagen. While high cellularity was observed in bone regions and integration zones between cartilage-allografts, recellularization of chondral implants was inconsistent, with cell migration typically less than ~750 µm into the dense decellularized tissue, possibly limiting long-term cellular maintenance. Our results demonstrate the structural and biomechanical efficacy of acellular allografts for at least six months in vivo.
关节软骨损伤后的修复具有挑战性，而脱细胞组织为促进再生提供了一种可能的治疗选择。在此，我们表明，在绵羊模型中，6个月后，与未治疗的缺损相比，脱细胞异体骨软骨移植可改善软骨的整合修复。通过MRI评估的组织内应变/结构的功能指标显示，植入物和天然软骨的生物力学相似。与天然组织和缺损相比，脱细胞异体移植物的结构、组成和摩擦学特性保留了表面粗糙度和润滑性、压缩和松弛状态下的材料特性、胶原蛋白：糖胺聚糖和胶原蛋白：磷酸盐的组成比例以及I/II型胶原蛋白的相对组成。虽然在骨区域和软骨异体移植物之间的整合区域观察到高细胞密度，但软骨植入物的再细胞化并不一致，细胞迁移通常小于约750 µm进入致密的脱细胞组织，这可能会限制长期细胞维持。我们的结果证明了脱细胞异体移植物在体内至少六个月的结构和生物力学功效。
