Department of Materials Science and Engineering, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei 10617, Taiwan.
Department of Applied Chemistry, National Yang Ming Chiao Tung University, 1001 University Road, Hsinchu 30010, Taiwan.
Langmuir. 2024 Oct 22;40(42):22417-22423. doi: 10.1021/acs.langmuir.4c03275. Epub 2024 Oct 9.
Antifouling properties are crucial for enhancing the longevity and functionality of biomedical implants, drug delivery systems, and biosensors. Zwitterionic polymers are renowned for their exceptional surface hydration and charge neutrality, which effectively resist biomolecular adsorption and protein attachment. We propose an innovative approach to develop zwitterion-like antifouling surfaces by chelating divalent cations with anionic poly(3,4-ethylenedioxythiophene) (PEDOT) films, specifically PEDOT-PO and PEDOT-COOH. The chelation behavior of these films was systematically evaluated using Na, Mg, and Ca ions. Divalent ions, particularly Ca and Mg, exhibit a strong affinity for the anionic groups, leading to significant antifouling properties. These modified surfaces effectively repelled both negatively charged bovine serum albumin (BSA) and positively charged lysozyme (LYZ) proteins across various pH environments. This study offers valuable insights into the antifouling characteristics of charged surfaces, enhancing our understanding of how ion-mediated surface modifications influence protein adsorption and interactions.
抗污特性对于提高生物医学植入物、药物输送系统和生物传感器的寿命和功能至关重要。两性离子聚合物以其出色的表面水合作用和电荷中性而闻名，可有效抵抗生物分子吸附和蛋白质附着。我们提出了一种通过螯合二价阳离子来开发类两性离子抗污表面的创新方法，具体来说是使用阴离子聚（3,4-亚乙基二氧噻吩）（PEDOT）薄膜，即 PEDOT-PO 和 PEDOT-COOH。使用 Na、Mg 和 Ca 离子系统地评估了这些薄膜的螯合行为。二价离子，特别是 Ca 和 Mg，对阴离子基团表现出很强的亲和力，从而具有显著的抗污特性。这些改性表面在各种 pH 环境下都能有效地排斥带负电荷的牛血清白蛋白 (BSA) 和带正电荷的溶菌酶 (LYZ) 蛋白。本研究深入了解了带电表面的抗污特性，增强了我们对离子介导的表面修饰如何影响蛋白质吸附和相互作用的理解。
