2018年，乌干达西南部一名发热患者未被诊断出的西尼罗河病毒2d谱系感染。

Undiagnosed West Nile virus lineage 2d infection in a febrile patient from South-west Uganda, 2018.

作者信息

Byaruhanga Timothy, Astbury Stuart, Hill Jack D, Tsoleridis Theocharis, Chappell Joseph G, Kayiwa John T, Ataliba Irene J, Nankya Annet M, Ball Jonathan K, Lutwama Julius J, McClure C Patrick

机构信息

School of Life Sciences, University of Nottingham, Nottingham, UK.

Uganda Virus Research Institute, Department of Arbovirology, Emerging and Re-emerging infectious diseases, Uganda.

出版信息

IJID Reg. 2024 Sep 26;13:100462. doi: 10.1016/j.ijregi.2024.100462. eCollection 2024 Dec.

DOI:10.1016/j.ijregi.2024.100462

PMID:39483153

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11525451/

Abstract

We report the retrospective identification and subsequent recovery of a near-complete West Nile Virus lineage 2 genomes from a hospitalized patient with acute febrile illness in Uganda, using a combination of degenerate primer polymerase chain reaction screening and a novel 1200bp nanopore-based whole-genome amplicon sequencing scheme. This represents the first West Nile virus genome to be recovered from a human in Uganda since its discovery in 1937. Basic molecular rather than serological surveillance methods could be more widely deployed in the region to better diagnose febrile infections.

摘要

我们报告了通过简并引物聚合酶链反应筛查和基于纳米孔的新型1200bp全基因组扩增子测序方案相结合的方法，从乌干达一名患有急性发热疾病的住院患者中回顾性鉴定并随后获得近乎完整的西尼罗河病毒2型基因组。这是自1937年西尼罗河病毒在乌干达被发现以来，首次从人类身上获得的该病毒基因组。在该地区可以更广泛地采用基础分子监测方法而非血清学监测方法，以更好地诊断发热性感染。