Dupeyron C M, Guillemin G A, Leluan G J
J Clin Pathol. 1986 Feb;39(2):208-11. doi: 10.1136/jcp.39.2.208.
A rapid method for diagnosing urinary tract infections, using identification and antimicrobial susceptibility testing that can be carried out in 24 hours, was devised. The method relies on direct inoculation of diluted urine (1/500) in the API 20 E and API ATB systems. Urine was simultaneously cultured on Columbia blood agar and on Drigalski agar to control the purity and for purposes of comparison. The results of this method and those obtained with a conventional method were compared by analysing 1352 urines. The results showed that all of the organisms were correctly identified using the conventional method, and susceptibility testing (rapid method) gave results that agreed with those of the classical method in 94% of cases, with major discrepancies in only 0.08% of cases. The rapid method applies only to monomicrobial infections.
设计了一种快速诊断尿路感染的方法，该方法采用鉴定和抗菌药敏试验，可在24小时内完成。该方法依靠将稀释尿液（1/500）直接接种于API 20 E和API ATB系统中。同时将尿液接种于哥伦比亚血琼脂和德里加尔斯基琼脂上，以控制纯度并用于比较。通过分析1352份尿液样本，对该方法的结果与传统方法所得结果进行了比较。结果显示，使用传统方法所有菌株均被正确鉴定，药敏试验（快速方法）在94%的病例中得出的结果与经典方法一致，仅有0.08%的病例存在重大差异。该快速方法仅适用于单一微生物感染。
