通过DNA菌落杂交法对人工污染食品中致病性小肠结肠炎耶尔森菌菌落进行计数。
Enumeration by DNA colony hybridization of virulent Yersinia enterocolitica colonies in artificially contaminated food.
作者信息
Jagow J, Hill W E
出版信息
Appl Environ Microbiol. 1986 Feb;51(2):441-3. doi: 10.1128/aem.51.2.441-443.1986.
Abstract
A genetic probe was used to identify and enumerate virulent Yersinia enterocolitica colonies in 11 artificially contaminated foods. Efficiency of enumeration, determined by autoradiography after DNA colony hybridization, ranged from 66 to 100% (average, 86%) and was influenced by the number of indigenous bacteria. The use of nitrocellulose filters and agar medium had little effect on efficiency of enumeration.
摘要
使用基因探针来鉴定和计数11种人工污染食品中有毒力的小肠结肠炎耶尔森菌菌落。通过DNA菌落杂交后的放射自显影确定的计数效率在66%至100%之间（平均为86%），并且受到本地细菌数量的影响。使用硝酸纤维素滤膜和琼脂培养基对计数效率影响不大。
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