Lu Yuxin, Guo Xuemei, Wang Xin, Zhang Xu, Wang Hongfei, Zhang Zhigang
Institute of Molecular Science, Chemical Biology and Molecular Engineering Laboratory of Education Ministry, Shanxi University, Taiyuan, 030006, Shanxi, China.
Biometals. 2025 Feb;38(1):285-295. doi: 10.1007/s10534-024-00654-6. Epub 2024 Dec 2.
Novel fluorescent superparamagnetic nanocomposites have been fabricated by introduction of the coumarin group on the surface of amine-functionalized magnetite-silica nanocomposites, and characterized by X-ray diffraction, Fourier transform infrared spectroscopy, transmission electron microscopy, fluorescence spectra, dynamic light scattering and vibrating sample magnetometer techniques. The nanocomposites were employed as delivery vehicles of a photoactive platinum diimine complex. The cellular uptake and photocytotoxicity of the photosensitizer-loaded nanocomposites in HeLa cells (human cervical cancer line) or HL-7702 cells (human liver cell line) have been studied by fluorescence spectra and cell viability assay, respectively. The results suggest that the nanocomposites can be used to monitor the cellular uptake of the photosensitizer, and can significantly enhance the photocytotoxicity of the photosensitizer towards cancer cells when employed as carriers of the photosensitizer. Also, the photosensitizer-loaded nanocomposites are almost nontoxic to human normal cells either in the dark or after irradiation.
通过在胺功能化的磁铁矿-二氧化硅纳米复合材料表面引入香豆素基团，制备了新型荧光超顺磁性纳米复合材料，并通过X射线衍射、傅里叶变换红外光谱、透射电子显微镜、荧光光谱、动态光散射和振动样品磁强计技术对其进行了表征。这些纳米复合材料被用作光活性铂二亚胺配合物的递送载体。分别通过荧光光谱和细胞活力测定法研究了负载光敏剂的纳米复合材料在HeLa细胞（人宫颈癌细胞系）或HL-7702细胞（人肝细胞系）中的细胞摄取和光细胞毒性。结果表明，纳米复合材料可用于监测光敏剂的细胞摄取，并且当用作光敏剂的载体时，可显著增强光敏剂对癌细胞的光细胞毒性。此外，负载光敏剂的纳米复合材料在黑暗中或照射后对人正常细胞几乎无毒。
