Liu Zhengjie, Yao Chuanjie, Xu Xingyuan, Huang Xinshuo, Huang Shuang, Zheng Shantao, Zhang Tao, Li Yan, Liu Fanmao, Wu Yuxiang, Liu Jing, Chen Hui-Jiuan, Xie Xi
State Key Laboratory of Optoelectronic Materials and Technologies, Guangdong Province Key Laboratory of Display Material and Technology, School of Electronics and Information Technology, Sun Yat-Sen University, Guangzhou, 510006, China.
School of Biomedical Engineering, Sun Yat-Sen University, Shenzhen, 518107, China.
Adv Sci (Weinh). 2025 Jun;12(24):e2409075. doi: 10.1002/advs.202409075. Epub 2024 Dec 16.
The real-time monitoring of in vivo electrophysiological and biochemical signals provides critical insights into the activities of tissues and organs. As the activity and metabolic state of different sites in the muscle vary, multichannel detection is necessary to capture the functional state of the whole muscle, yet the access to the bio-information in subcutaneous space remained challenging. This work reports the development of a reconfigurable microneedle electrode array integrated system designed to achieve painless and minimally invasive monitoring of subcutaneous electromyogram (EMG), oxygen species, and pH through an array of thumbtack-shaped microneedle (TSMN) electrode. By assembling discrete TSMNs into an array, the system enables multi-parameter detection with single microneedle resolution. The PEDOT: PSS layer is electrochemically deposited on the TSMNs, enhancing their signal-sensing capabilities and electrochemical properties. Additionally, the design of the pogo pin interface ensures reliable signal transmission and stable device performance, while allowing flexible replacement of the TSMNs, which enhances system maintainability and longevity. Validation experiments conducted on in vivo animal models demonstrate the system's capability in real-time monitoring of muscle fatigue and indicators related to sciatic nerve injury. These results advance the development of wearable technologies for monitoring subcutaneous physiological and biochemical information for diagnosing neuromuscular disorders.
体内电生理和生化信号的实时监测为了解组织和器官的活动提供了关键见解。由于肌肉中不同部位的活动和代谢状态各不相同，因此需要进行多通道检测以获取整个肌肉的功能状态，然而，获取皮下空间中的生物信息仍然具有挑战性。这项工作报告了一种可重构微针电极阵列集成系统的开发，该系统旨在通过一系列图钉形微针（TSMN）电极实现对皮下肌电图（EMG）、氧种类和pH值的无痛、微创监测。通过将离散的TSMN组装成阵列，该系统能够以单微针分辨率进行多参数检测。聚（3,4-乙撑二氧噻吩）：聚苯乙烯磺酸盐（PEDOT:PSS）层通过电化学沉积在TSMN上，增强了它们的信号传感能力和电化学性能。此外，弹簧针接口的设计确保了可靠的信号传输和稳定的设备性能，同时允许灵活更换TSMN，这提高了系统的可维护性和使用寿命。在体内动物模型上进行的验证实验证明了该系统实时监测肌肉疲劳和与坐骨神经损伤相关指标的能力。这些结果推动了用于监测皮下生理和生化信息以诊断神经肌肉疾病的可穿戴技术的发展。
