Zhang Qianyi, Sun Bing, Shanehsazzadeh Saeed, Bongers Andre, Gu Zi
School of Chemical Engineering, University of New South Wales, Sydney, NSW 2052, Australia.
School of Chemistry, University of Sydney, Sydney, NSW 2006, Australia.
Nanomaterials (Basel). 2024 Dec 7;14(23):1964. doi: 10.3390/nano14231964.
Engineering magnetic nanoparticles with tunable structural properties and magnetism is critical to develop desirable magnetic particle imaging (MPI) tracers for biomedical applications. Here we present a new superparamagnetic metal oxide nanoparticle with a controllable chemical composition and magnetism for imaging tumor xenografts in living mice. Superparamagnetic Zn/Fe mixed metal oxide (ZnFe-MMO) nanoparticles are fabricated via a facile one-pot co-precipitation method in water followed by thermal decomposition with tunable Zn/Fe ratios and at various calcination temperatures. This work, for the first time, presented LDH-derived metal oxides for an MPI application. The metal composition is tunable to present an optimized MPI performance. The analytical results demonstrate that ZnFe-MMO nanoparticles at the designed molar ratio of Zn/Fe = 2:1 after 650 °C calcination demonstrate a higher saturation magnetization (M) value and optimal MPI signal than the samples presented with other conditions. The excellent biocompatibility of ZnFe-MMO is demonstrated in both breast cancer cells and fibroblast cell cultures. In vivo imaging of 4T1 tumor xenografts in mice using ZnFe-MMO as a tracer showed that the mean signal intensity is 1.27-fold higher than the commercial tracer VivoTrax at 72 h post-injection, indicating ZnFe-MMO's promise for prolonged MPI imaging applications.
设计具有可调结构特性和磁性的磁性纳米颗粒对于开发适用于生物医学应用的理想磁性粒子成像（MPI）示踪剂至关重要。在此，我们展示了一种新型超顺磁性金属氧化物纳米颗粒，其具有可控的化学成分和磁性，用于对活体小鼠中的肿瘤异种移植进行成像。通过在水中简便的一锅共沉淀法制备超顺磁性Zn/Fe混合金属氧化物（ZnFe-MMO）纳米颗粒，随后在不同的Zn/Fe比和各种煅烧温度下进行热分解。这项工作首次展示了源自层状双氢氧化物（LDH）的金属氧化物用于MPI应用。金属成分可调节以呈现优化的MPI性能。分析结果表明，在650°C煅烧后，设计的Zn/Fe摩尔比为2:1的ZnFe-MMO纳米颗粒比其他条件下的样品表现出更高的饱和磁化强度（M）值和最佳的MPI信号。ZnFe-MMO在乳腺癌细胞和成纤维细胞培养中均表现出优异的生物相容性。以ZnFe-MMO作为示踪剂对小鼠体内4T1肿瘤异种移植进行成像显示，在注射后72小时，平均信号强度比商业示踪剂VivoTrax高1.27倍，表明ZnFe-MMO在延长MPI成像应用方面具有潜力。
