Chen Linji, Mori Yusaku, Nishii Shogo, Sakamoto Miwa, Ohara Makoto, Yamagishi Sho-Ichi, Sekizawa Akihiko
Department of Obstetrics and Gynecology, Showa University Graduate School of Medicine, 1-5-8 Hatanodai, Shinagawa, Tokyo 142-8555, Japan.
Department of Diabetes, Metabolism, and Endocrinology, Showa University Graduate School of Medicine, 1-5-8 Hatanodai, Shinagawa, Tokyo 142-8555, Japan.
J Clin Med. 2024 Nov 26;13(23):7158. doi: 10.3390/jcm13237158.
: Male factors contribute to approximately 50% of infertile couples. However, obvious causes remain unknown in many cases. This observational study aimed to investigate the associations of clinical and lifestyle parameters with sperm parameters. : This study enrolled 41 men in infertile couples without obvious causes for male infertility from July 2023 to April 2024. Semen samples were evaluated for sperm number, motility, DNA fragmentation, and oxidative stress (OS) marker oxidation-reduction potential (ORP). Blood samples were analyzed for biochemical parameters, including advanced glycation end products (AGEs), and systemic OS marker diacron-reactive oxygen metabolites (d-ROMs). Skin-accumulated AGE levels were identified with an autofluorescence method. Lifestyle factors were assessed with a lifestyle questionnaire. : Most of the participants were under 40 years old and non-obese with normal clinical parameters. Multiple regression analyses revealed that body mass index, serum d-ROMs, and semen ORP levels were independently associated with decreased sperm number. Additionally, serum zinc and semen ORP levels were associated with sperm motility. Furthermore, serum zinc and high-density lipoprotein cholesterol levels were associated with sperm progressive motility and DNA fragmentation, respectively. The rest of the clinical and lifestyle factors, including skin-accumulated and serum AGE levels, were not correlated with any sperm parameters. Furthermore, serum d-ROM and semen ORP levels were not correlated with each other or any of the clinical and lifestyle factors. : Our present study indicates that both systemic and local OS may be independently involved in sperm abnormality in healthy men without obvious causes for male infertility.
男性因素导致约50%的不育夫妇不育。然而，在许多情况下，明显的病因仍然不明。这项观察性研究旨在调查临床和生活方式参数与精子参数之间的关联。
本研究纳入了2023年7月至2024年4月期间41对不育夫妇中无明显男性不育病因的男性。对精液样本进行精子数量、活力、DNA碎片和氧化应激（OS）标志物氧化还原电位（ORP）评估。对血液样本进行生化参数分析，包括晚期糖基化终末产物（AGEs）和全身OS标志物二亚铬反应性氧代谢产物（d-ROMs）。采用自体荧光法测定皮肤累积AGE水平。通过生活方式问卷评估生活方式因素。
大多数参与者年龄在40岁以下，非肥胖，临床参数正常。多元回归分析显示，体重指数、血清d-ROMs和精液ORP水平与精子数量减少独立相关。此外，血清锌和精液ORP水平与精子活力相关。此外，血清锌和高密度脂蛋白胆固醇水平分别与精子前向运动和DNA碎片相关。其余的临床和生活方式因素，包括皮肤累积和血清AGE水平，与任何精子参数均无相关性。此外，血清d-ROM和精液ORP水平彼此之间以及与任何临床和生活方式因素均无相关性。
我们目前的研究表明，全身和局部OS可能独立参与无明显男性不育病因的健康男性的精子异常。
