Zhang Ying, Yin Hang
School of Pharmaceutical Sciences, Key Laboratory of Bioorganic Phosphorous chemistry and Chemical Biology(Ministry of Education), Tsinghua University, Beijing 100084, China.
Tsinghua-Peking Center for Life Sciences, Tsinghua University, Beijing 100084, China.
Extracell Vesicles Circ Nucl Acids. 2022 Apr 13;3(2):87-92. doi: 10.20517/evcna.2022.16. eCollection 2022.
Extracellular vesicles (EVs) have been regarded as influential intracellular delivering parcels that possess tremendous potential because of their strict and complex secretion regulation processes. However, traditional detection methods cannot monitor the secretion of EVs due to their small particle diameters. Inspired by their peculiar diverse appearances and lipid membranes ingredients, we developed an innovative strategy to detect EVs in any kind of fluids by using rationally designed peptide probes that particularly recognize the highly curved surface of EVs. These peptide probes also serve as novel tools to selectively target cancerous cells with specific lipid compositions and distributions. With this strategy, we discovered a series of EV-secreting regulation mechanisms and identified their roles within physiological processes. Recently, we found that the transportation of oligodeoxynucleotides and cell division control protein 42 homolog from TLR9-activated macrophages to naïve cells via EVs exerts synergetic effects in the propagation of the intracellular immune response, which suggests a general mechanism for EV-mediated uptake of pathogen-associated molecular patterns.
细胞外囊泡（EVs）因其严格且复杂的分泌调控过程，被视为具有巨大潜力的重要细胞内传递包裹。然而，由于其粒径小，传统检测方法无法监测EVs的分泌。受其独特多样的外观和脂质膜成分的启发，我们开发了一种创新策略，通过使用合理设计的肽探针来检测任何类型液体中的EVs，这些肽探针能特异性识别EVs的高度弯曲表面。这些肽探针还可作为新型工具，选择性地靶向具有特定脂质组成和分布的癌细胞。通过这一策略，我们发现了一系列EVs分泌调控机制，并确定了它们在生理过程中的作用。最近，我们发现寡脱氧核苷酸和细胞分裂控制蛋白42同源物从TLR9激活的巨噬细胞通过EVs转运至幼稚细胞，在细胞内免疫反应的传播中发挥协同作用，这提示了一种EV介导的病原体相关分子模式摄取的普遍机制。
