Isolation and Molecular Identification of - and -producing Genes from Maize Feed Contaminated with Fungus in Silos of Dairy Farms in Fars province, Iran.

Mycotoxins are toxins produced by various types of fungi, including , which can produce different types of mycotoxins, such as Deoxynivalenol (DON), Zearalenone, T-2 toxin, and Fumonisins (FUM). Mycotoxins have the potential to reduce the quality of crops and pose health risks to both humans and animals. This can result in reduced animal production and substantial economic consequences on a global scale. Extensive research has been carried out to investigate the high incidence of contamination in grains by fungi. In this study, 80 samples of maize from silos of dairy farms in Fars province, Iran were collected and examined for fungal contamination by and potential production of DON and FUM. For this purpose, identification using specific primers for different genes was carried out by polymerase chain reaction test and gel electrophoresis with agarose (1%). Among different counties, the silos in Kharameh with 47.05% and Jahrom with 46.15% had the highest contamination rates, and the lowest contamination rate belonged to Kazeroon with 27.27%. Out of the 30 positive samples contaminated by , 21 produced FUM, 4 produced DON, and 5 produced both toxins. species that contaminated the maize samples were also identified, including 13, 10, and 7 samples contaminated by , , and , respectively. As a conclusion, the findings of the study indicated that 37.5% of the corn samples from dairy farm silos in Fars province were contaminated with the fungus, which had the potential to produce the toxic mycotoxins, deoxynivalenol, and fumonisins.