雕翅隧蜂（膜翅目，蜜蜂总科，切叶蜂科）的染色体水平基因组组装

Chromosome-level genome assembly of Megachile sculpturalis Smith (Hymenoptera, Apoidea, Megachilidae).

作者信息

Yang Guang, Zhou Qingsong, Yang Juanjuan, Chen Guoai, Niu Zeqing, Orr Michael, Ferrari Rafael Rodrigues, Zhang Yihan, Shi Xiaoyu, Cheng Rui, Zhu Chaodong, Luo Arong

机构信息

Key Laboratory of Zoological Systematics and Evolution, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.

College of Biological Sciences, University of Chinese Academy of Science, Beijing, China.

出版信息

Sci Data. 2025 Jan 10;12(1):46. doi: 10.1038/s41597-025-04385-2.

DOI:10.1038/s41597-025-04385-2

PMID:39794368

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11724062/

Abstract

Megachile sculpturalis Smith, 1853 native to East Asia, is an important solitary bee species that has invaded both Europe and the United States. This study provides the first chromosome-level genome assembly of M. sculpturalis using a combination of Nanopore long reads, Illumina short reads, and Hi-C data. The genome comprises 296.99 Mb distributed across 16 chromosomes. N50, L50 and BUSCO completeness reached 19.128 Mb, 7 scaffolds, and 96.7%, respectively. The genome contains 104 Mb repetitive elements (35.02% of the assembly size) and 11,446 predicted protein-coding genes. This chromosome-level genome will serve as an essential genomic resource for future research on Megachilidae.

摘要

1853年的雕木蜂（Megachile sculpturalis Smith）原产于东亚，是一种已入侵欧洲和美国的重要独居蜂物种。本研究结合纳米孔长读长、Illumina短读长和Hi-C数据，首次提供了雕木蜂的染色体水平基因组组装。该基因组由分布在16条染色体上的2.9699亿碱基对组成。N50、L50和BUSCO完整性分别达到1912.8万碱基对、7个支架和96.7%。该基因组包含1.04亿碱基对的重复元件（占组装大小的35.02%）和11446个预测的蛋白质编码基因。这个染色体水平的基因组将作为未来切叶蜂科研究的重要基因组资源。