Filion L G, Cho H J, Shewen P E, Raybould T J, Wilkie B N
Can J Comp Med. 1985 Jan;49(1):99-103.
Analysis of 45 sera was performed employing five techniques which are currently in use in three laboratories to measure anti-Pasteurella haemolytica antibodies. The enzyme linked immunosorbent assay, passive hemagglutination, complement fixation and direct and indirect bacterial agglutination assays were employed and a relationship between tests in the measurement of anti-P. haemolytica antibodies was demonstrated. Regression analysis together with prediction and confidence intervals were tabulated also. The conclusion drawn from statistical analysis was that all five tests are similar in their ability to detect immune responses (antibody and antigen(s) interactions) to Pasteurella haemolytica.
利用目前三个实验室正在使用的五种技术对45份血清进行分析,以检测抗溶血巴斯德氏菌抗体。采用了酶联免疫吸附测定、被动血凝、补体结合以及直接和间接细菌凝集测定法,并证明了这些检测方法在抗溶血巴斯德氏菌抗体测量中的相关性。还列出了回归分析以及预测和置信区间。统计分析得出的结论是,所有这五种检测方法在检测对溶血巴斯德氏菌的免疫反应(抗体与抗原相互作用)的能力方面相似。