Robins-Browne R M, Still C S, Miliotis M D, Koornhof H J
Infect Immun. 1979 Aug;25(2):680-4. doi: 10.1128/iai.25.2.680-684.1979.
Enterotoxin derived from three clinical isolates of Yersinia enterocolitica was compared with the heat-stable enterotoxin of Escherichia coli. Both toxins were biologically active in infant mice examined at 2 h and in ligated rabbit ileal loops at 6 h. Neither substance, however, produced changes in ligated ileal loops at 18 h or in Chinese hamster ovary or Y1 adrenal tissue cultures. In addition, both Y. enterocolitica enterotoxin concentrated approximately 20 times by ammonium sulfate precipitation and ultrafiltration and a similarly prepared sample of E. coli heat-stable enterotoxin stimulated the activity of guanylate cyclase but not that of adenylate cyclase in infant mouse intestine. These findings suggest that the role of enterotoxin in the pathogenesis of intestinal Y.enterocolitica infection may be similar to that of heat-stable enterotoxin in E. coli diarrhea.
对源自小肠结肠炎耶尔森氏菌三个临床分离株的肠毒素与大肠杆菌的热稳定肠毒素进行了比较。两种毒素在2小时时检测的幼鼠以及6小时时结扎的兔回肠袢中均具有生物活性。然而，这两种物质在18小时时均未使结扎的回肠袢产生变化，在中国仓鼠卵巢或Y1肾上腺组织培养物中也未产生变化。此外，通过硫酸铵沉淀和超滤浓缩约20倍的小肠结肠炎耶尔森氏菌肠毒素以及同样制备的大肠杆菌热稳定肠毒素样品，均刺激了幼鼠肠道中鸟苷酸环化酶的活性，但未刺激腺苷酸环化酶的活性。这些发现表明，肠毒素在小肠结肠炎耶尔森氏菌肠道感染发病机制中的作用可能与热稳定肠毒素在大肠杆菌腹泻中的作用相似。
