通过乳鼠生物测定法检测小肠结肠炎耶尔森菌热稳定肠毒素。
Detection of Yersinia enterocolitica heat-stable enterotoxin by suckling mouse bioassay.
作者信息
Nunes M P, Ricciardi I D
出版信息
J Clin Microbiol. 1981 Apr;13(4):783-6. doi: 10.1128/jcm.13.4.783-786.1981.
Abstract
A comparison of heat-stable enterotoxins from Escherichia coli and Yersinia enterocolitica by the suckling mouse bioassay showed that whereas E. coli heat-stable enterotoxin evoked a maximum ratio of gut weight to remaining body weight after a 4-h incubation period, the maximum ratio for Y. enterocolitica was achieved at 2 h, decreasing thereafter to values regarded as negative (less than 0.083). This action of Y. enterocolitica heat-stable enterotoxin may give false-negative results for the standard suckling mouse bioassay.
摘要
通过乳鼠生物测定法对大肠杆菌和小肠结肠炎耶尔森氏菌的热稳定肠毒素进行比较，结果显示，大肠杆菌热稳定肠毒素在孵育4小时后会引起肠道重量与剩余体重的最大比值，而小肠结肠炎耶尔森氏菌的最大比值在2小时时达到，此后逐渐下降至被视为阴性的值（小于0.083）。小肠结肠炎耶尔森氏菌热稳定肠毒素的这种作用可能会导致标准乳鼠生物测定法出现假阴性结果。
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