Liu Yi-Shan, Lai Mei Chou, Hong Tang-Yao, Liu I-Min
Department of Dermatology, E-Da Hospital, I-Shou University, Kaohsiung City, 84001, Taiwan.
School of Chinese Medicine for Post Baccalaureate, College of Medicine, I-Shou University, Kaohsiung City, 84001, Taiwan.
Heliyon. 2025 Feb 7;11(4):e42551. doi: 10.1016/j.heliyon.2025.e42551. eCollection 2025 Feb 28.
γ-Oryzanol (Orz) is a powerful antioxidant found in rice bran and oil, known for its numerous health benefits. Nevertheless, its precise impact on skin wound healing remains largely unexplored. This research focuses on comprehensively examining the ability of Orz to promote tissue repair through laboratory-based and animal model experiments. The research further examines its antimicrobial activity and its role in promoting wound healing through anti-inflammatory effects. To evaluate the effect of Orz on tissue repair, scratch assays performed were used to analyze its impact on cellular migration. An experiment utilizing a rat skin wound excision model was carried out to investigate wound contraction rates and analyze histological modifications. The granulation tissue from a dead space wound model was examined to assess the levels of inflammatory mediators, free radicals, and antioxidant activity. The findings of the study demonstrated Orz improves cellular viability while facilitating the proliferation and migration of NIH-3T3 cells. It further promotes the generation of growth factors, such as fibroblast growth factor 21 and transforming growth factor-β. Moreover, Orz exhibited significant antimicrobial activity against various pathogens commonly found in wounds. An ointment with 10 % Orz showed significant effectiveness in promoting wound healing, as indicated by notable wound contraction observed on the 14th day after surgery. Histological analysis demonstrated that the application of 10 % Orz ointment resulted in remarkable tissue repair, heightened fibroblast proliferation, and improved formation of new blood vessels. The application of Orz ointment was also found to elevate interleukin-10 levels, reduce tumor necrosis factor-α levels and ED-1-immunopositive cells, and enhance antioxidant enzyme activity, thereby mitigating oxidative damage in healing tissues throughout the initial stages of tissue repair. These results indicate that Orz holds significant promise for application in the treatment of surface wounds.
γ-谷维素（Orz）是一种存在于米糠和米糠油中的强效抗氧化剂，因其众多的健康益处而闻名。然而，其对皮肤伤口愈合的确切影响在很大程度上仍未得到探索。本研究聚焦于通过基于实验室和动物模型的实验全面考察Orz促进组织修复的能力。该研究进一步考察其抗菌活性以及通过抗炎作用在促进伤口愈合中的作用。为评估Orz对组织修复的影响，采用划痕试验分析其对细胞迁移的影响。利用大鼠皮肤伤口切除模型进行实验，以研究伤口收缩率并分析组织学变化。检查死腔伤口模型的肉芽组织，以评估炎症介质、自由基水平和抗氧化活性。研究结果表明，Orz可提高细胞活力，同时促进NIH-3T3细胞的增殖和迁移。它还能促进生长因子的产生，如成纤维细胞生长因子21和转化生长因子-β。此外，Orz对伤口中常见的各种病原体表现出显著的抗菌活性。含10%Orz的软膏在促进伤口愈合方面显示出显著效果，术后第14天观察到明显的伤口收缩即表明了这一点。组织学分析表明，应用10%Orz软膏可实现显著的组织修复、增强成纤维细胞增殖并改善新血管形成。还发现应用Orz软膏可提高白细胞介素-10水平、降低肿瘤坏死因子-α水平和ED-1免疫阳性细胞数量，并增强抗氧化酶活性，从而在组织修复的初始阶段减轻愈合组织中的氧化损伤。这些结果表明，Orz在治疗体表伤口方面具有巨大的应用潜力。
