GöktürkBaydar Nilgün, Babalik Zehra, Demirci Tunahan, Cessur Alper
Department of Agricultural Biotechnology, Faculty of Agriculture, Isparta University of Applied Sciences, Isparta, 32270, Turkey.
Department of Plant and Animal Production, Atabey Vocational School, Isparta University of Applied Sciences, Atabey-Isparta, 32670, Turkey.
Sci Rep. 2025 Mar 5;15(1):7667. doi: 10.1038/s41598-025-85545-7.
Silver nanoparticles (AgNPs) are one of nanoparticles with promising applications in various fields due to their unique characteristics. This study was carried out to determine the effects of AgNPs obtained by different green syntheses procedures on their characteristic properties and the accumulation of phenolic compounds in cell suspension cultures of Kalecik Karası grape cultivar. AgNPs were obtained by 24 different green synthesis methods including modifications in extraction method, reaction pH and conditions. When the results of the analyses conducted to determine the structural properties of AgNPs are evaluated, it was observed that more spherical and smaller nanoparticles were synthesized under alkaline conditions. The smallest NP size was detected as 8.9 nm in NP11, while the largest NP size (59.6 nm) was found in NP19. AgNPs obtained at room conditions for 4 h and pH 7 significantly increased the total phenolic, trans-resveratrol, catechin and epicatechin contents, while water or methanol extracts used in the synthesis had no significant effect. As a result of the study, it was determined that not only the characteristic properties of AgNPs but also their effectiveness on the secondary metabolite production varied significantly depending on the extraction method, pH and conditions of the reaction solution during synthesis.
银纳米颗粒（AgNPs）因其独特特性而成为在各个领域具有广阔应用前景的纳米颗粒之一。本研究旨在确定通过不同绿色合成方法获得的AgNPs对其特性以及Kalecik Karası葡萄品种细胞悬浮培养物中酚类化合物积累的影响。AgNPs通过24种不同的绿色合成方法获得，包括提取方法、反应pH值和条件的改变。在评估用于确定AgNPs结构特性的分析结果时，观察到在碱性条件下合成出更多球形且更小的纳米颗粒。在NP11中检测到最小的纳米颗粒尺寸为8.9纳米，而在NP19中发现最大的纳米颗粒尺寸（59.6纳米）。在室温条件下反应4小时且pH值为7时获得的AgNPs显著增加了总酚、反式白藜芦醇、儿茶素和表儿茶素的含量，而合成中使用的水或甲醇提取物则没有显著影响。研究结果表明，不仅AgNPs的特性，而且其对次生代谢产物产生的有效性在很大程度上取决于合成过程中的提取方法、反应溶液的pH值和条件。
