Wu Yanhong, Yu Min, Zhang Zenghu, Wang Yanan, Yang Xiaoting, Wang Xiaolei, Ren Qiaomeng, Sun Zhongcheng, Shi Xiaochong, Zhang Xiao-Hua
College of Marine Life Sciences, Ocean University of China, Qingdao 266003, PR China.
Institute of Evolution and Marine Biodiversity, Ocean University of China, Qingdao 266003, PR China.
Int J Syst Evol Microbiol. 2017 Sep;67(9):3203-3208. doi: 10.1099/ijsem.0.002082. Epub 2017 Aug 22.
A Gram-staining-negative, yellow-colony-forming, rod-shaped, non-flagellated and facultatively aerobic strain, designed HRA130-1T, was isolated from a deep-sea polymetallic nodule from the Pacific Clarion-Clipperton Fracture Zone (CCFZ). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain HRA130-1T belonged to the genus Polaribacter (96.3-93.2 % 16S rRNA gene sequence similarity), and exhibited 94 % 16S rRNA gene sequence similarity to Polaribacter filamentus KCTC 23135T (type species) and the highest sequence similarity to Polaribacter huanghezhanensis KCTC 32516T (96.3 %). Optimal growth occurred in the presence of 4 % (w/v) NaCl, at pH 7.0 and 16 °C. The DNA G+C content of strain HRA130-1T was 35.9 mol%. The major fatty acid was iso-C15 : 0. The predominant respiratory quinone was menaquinone-6 (MK-6). The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, one unidentified phospholipid and an unidentified aminolipid. On the basis of data from the present taxonomic study using a polyphasic approach, strain HRA130-1T represents a novel species of the genus Polaribacter, for which the name Polaribacter pacificus sp. nov. is proposed. The type strain is HRA130-1T (=KCTC 52370T=MCCC 1K03199T=JCM 31460T=CGMCC 1.15763T).
从太平洋克拉里昂-克利珀顿断裂带（CCFZ）的深海多金属结核中分离出一株革兰氏染色阴性、形成黄色菌落、杆状、无鞭毛且兼性需氧的菌株，命名为HRA130-1T。基于16S rRNA基因序列的系统发育分析表明，菌株HRA130-1T属于极地杆菌属（16S rRNA基因序列相似性为96.3-93.2%），与丝状极地杆菌KCTC 23135T（模式种）的16S rRNA基因序列相似性为94%，与黄河站极地杆菌KCTC 32516T的序列相似性最高（96.3%）。在4%（w/v）NaCl、pH 7.0和16°C条件下生长最佳。菌株HRA130-1T的DNA G+C含量为35.9 mol%。主要脂肪酸为异C15:0。主要呼吸醌为甲基萘醌-6（MK-6）。主要极性脂为磷脂酰乙醇胺、磷脂酰甘油、一种未鉴定的磷脂和一种未鉴定的氨基脂。基于本多相分类研究的数据，菌株HRA130-1T代表极地杆菌属的一个新物种，为此提出名为太平洋极地杆菌（Polaribacter pacificus）的新种。模式菌株为HRA130-1T（=KCTC 52370T=MCCC 1K03199T=JCM 31460T=CGMCC 1.15763T）。
