Pollack M, Taylor N S, Callahan L T
Infect Immun. 1977 Mar;15(3):776-80. doi: 10.1128/iai.15.3.776-780.1977.
Seventy-five consecutive clinical Pseudomonas aeruginosa isolates were tested for in vitro exotoxin production. Exotoxin was demonstrated in culture filtrates biologically, by its ability to produce characteristic dermonecrotic lesions in guinea pigs, and seriologically, by counterimmunoelectrophoresis (CIE) with rabbit antiserum elicited with purified exotoxin. By these two methods, exotoxin was detected in 87 and 89% of P. aeruginosa strains, respectively (r = 0.48, P less than 0.001). Although less sensitive than CIE in detecting exotoxin immunodiffusion demonstrated a reaction of antigenic identity in most cases. Exotoxin was produced by all seven Fisher-Devlin immunotypes and by untypable strains. In contrast, exotoxin was not detected in the culture filtrates of 16 non-aeruginosa pseudomonas isolates and 48 non-pseudomonas organisms. The production of biologically similar antigenically closely related exotoxins is thus a characteristic of the majority of P. aeruginosa strains derived from diverse clinical sources.
对75株连续分离得到的临床铜绿假单胞菌菌株进行了体外毒素产生情况的检测。通过其在豚鼠体内产生特征性皮肤坏死病变的能力，从生物学角度在培养滤液中证实了外毒素的存在；通过用纯化外毒素诱导产生的兔抗血清进行对流免疫电泳（CIE），从血清学角度证实了外毒素的存在。通过这两种方法，分别在87%和89%的铜绿假单胞菌菌株中检测到了外毒素（r = 0.48，P < 0.001）。虽然在检测外毒素方面不如CIE敏感，但免疫扩散在大多数情况下显示出抗原同一性反应。所有七种费希尔 - 德夫林免疫型以及不可分型菌株均产生外毒素。相比之下，在16株非铜绿假单胞菌假单胞菌分离株和48株非假单胞菌生物体的培养滤液中未检测到外毒素。因此，产生生物学上相似且抗原关系密切的外毒素是大多数来自不同临床来源的铜绿假单胞菌菌株的一个特征。
