Putzrath R M, Maniloff J
J Virol. 1977 May;22(2):308-14. doi: 10.1128/JVI.22.2.308-314.1977.
The growth of an enveloped DNA-containing mycoplasmavirus (MVL2 obtained from R.N. Gourlay) has studied, by using the indicator host Acholeplasma laidlawii strain JA1. From virus one-step growth curves, artificial lysis experiments, and infected cell growth curves, it was found that virus infection is nonlytic. Newly infected cells grow slower and are osmotically more stable than uninfected cells. However, 4 to 6 h after infection, the cells reach a carrier state in which cell growth rate and osmotic fragility are indistinguishable from uninfected cells. Carrier cultures contain free virus. Every carrier culture cell gives rise to either a clone of carrier cells or a clone of MVL2-resistant cells.
利用指示宿主莱氏无胆甾原体JA1株，对一种含包膜的DNA支原体病毒（从R.N. 古尔利获得的MVL2）的生长进行了研究。从病毒一步生长曲线、人工裂解实验以及感染细胞生长曲线发现，病毒感染是非裂解性的。新感染的细胞生长较慢，并且在渗透压方面比未感染的细胞更稳定。然而，感染后4至6小时，细胞进入携带状态，此时细胞生长速率和渗透脆性与未感染的细胞无法区分。携带培养物中含有游离病毒。每个携带培养细胞要么产生携带细胞克隆，要么产生MVL2抗性细胞克隆。
