由harpin蛋白激活的不同免疫途径在……中
Distinct immune pathways activated by harpin proteins in .
作者信息
Patoliya Jaimini, Thaker Khushali, Rabadiya Khushbu, Patel Jalpa, Trivedi Tithi, Patel Saumya, Jain Nayan, Kumar Prasant, Joshi Rushikesh
机构信息
Department of Biochemistry and Forensic Sciences, University School of Sciences, Gujarat University, Ahmedabad, 380009 India.
Department of Microbiology and Biotechnology, University School of Sciences, Gujarat University, Ahmedabad, 380009 India.
出版信息
Physiol Mol Biol Plants. 2025 May;31(5):785-793. doi: 10.1007/s12298-025-01601-6. Epub 2025 Jun 3.
UNLABELLED
This study investigates how two harpin proteins, HrpZ and Hpa1, modulate plant immunity as potential alternatives to pesticides. Despite the established importance of SA in immunity, its regulatory complexity and gene expression dynamics are partially elucidated. We examined the effect of HrpZ and Hpa1 on the SA signalling pathway and the expression of seven marker genes (, , , , , , ) identified through existing microarray dataset analysis and analysed by qRT-PCR and HPLC. Our findings reveal distinct modes of action: HrpZ strongly activates SA-mediated immunity by upregulating SA levels and SA-responsive genes. Conversely, Hpa1 does not significantly impact SA levels but upregulates and , suggesting an alternative, SA-independent defence mechanism. This research provides valuable insights into harpin-induced plant immunity, paving the way for targeted biostimulant development in sustainable agriculture.
SUPPLEMENTARY INFORMATION
The online version contains supplementary material available at 10.1007/s12298-025-01601-6.
未标注
本研究调查了两种过敏蛋白HrpZ和Hpa1如何作为农药的潜在替代品调节植物免疫力。尽管水杨酸（SA）在免疫中已确立其重要性，但其调控复杂性和基因表达动态仍部分有待阐明。我们通过现有的微阵列数据集分析鉴定了七个标记基因（、、、、、、），并通过qRT-PCR和HPLC检测了HrpZ和Hpa1对SA信号通路及这些基因表达的影响。我们的研究结果揭示了不同的作用模式：HrpZ通过上调SA水平和SA响应基因来强烈激活SA介导的免疫。相反，Hpa1对SA水平没有显著影响，但上调了和，表明存在一种独立于SA的替代防御机制。本研究为过敏蛋白诱导的植物免疫提供了有价值的见解，为可持续农业中有针对性的生物刺激剂开发铺平了道路。
补充信息
在线版本包含可在10.1007/s12298-025-01601-6获取的补充材料。
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