Gunnarsdottir Sissy Häsler, Rehrl Johanna, Sepperer Thomas, Haretzmüller Lena, Sesink David, Löwenstein Katja, Gadermayr Michael, Hölzl Claudia, Stelzhammer Bettina, Himmelsbach Markus, Oostingh Gertie Janneke, Schnabel Thomas, Schuster Anja
Department of Health Sciences, Salzburg University of Applied Sciences, Urstein Süd 1, 5412, Puch bei Hallein, Austria.
Department of Design and Green Engineering, Salzburg University of Applied Sciences, Markt 136a, 5431, Kuchl, Austria.
Sci Rep. 2025 Jul 1;15(1):21340. doi: 10.1038/s41598-025-06105-7.
The growing interest in plant-based therapeutics has increased investigations into natural resources rich in bioactive compounds, such as tree bark. Despite historical use in traditional medicine, the therapeutic potential of European tree bark remains underexplored. This study evaluated the antioxidant, antimicrobial, and wound-healing properties of aqueous extracts from the bark of six European tree species, namely Alnus glutinosa, Fagus sylvatica, Betula pendula, Prunus padus, Quercus spp., and Pinus sylvestris. The bark extracts were analyzed for chemical composition, total phenolic content, and antioxidative properties using a DPPH assay and a ROS inhibition assay in human keratinocytes. Antimicrobial activity was tested against seven bacterial strains using a MIC assay, and the wound healing potential was evaluated using a scratch assay. All extracts enhanced wound closure and decreased ROS production in HaCaT cells, with alder and bird cherry bark extracts showing the strongest effects. The antimicrobial assay revealed potent activity against Gram-positive pathogens, with alder bark extract showing the greatest efficacy. The findings highlight the potential therapeutic application of European tree bark extracts in addressing oxidative stress, infections, and wound healing, validating traditional medicinal practices, and providing a sustainable approach for forestry byproducts in modern phytotherapy, potentially contributing to skin disorder treatments.
人们对植物性疗法的兴趣日益浓厚，这促使对富含生物活性化合物的自然资源（如树皮）的研究不断增加。尽管树皮在传统医学中有过应用历史，但欧洲树皮的治疗潜力仍未得到充分探索。本研究评估了六种欧洲树种（即欧洲桤木、欧洲水青冈、垂枝桦、稠李、栎属树种和欧洲赤松）树皮水提取物的抗氧化、抗菌和伤口愈合特性。通过DPPH法和人角质形成细胞中的ROS抑制试验，对树皮提取物的化学成分、总酚含量和抗氧化性能进行了分析。使用MIC试验对七种细菌菌株进行抗菌活性测试，并使用划痕试验评估伤口愈合潜力。所有提取物均能促进HaCaT细胞的伤口闭合并减少ROS产生，其中桤木和稠李树皮提取物的效果最为显著。抗菌试验显示对革兰氏阳性病原体具有强大活性，桤木树皮提取物的疗效最为显著。这些发现突出了欧洲树皮提取物在应对氧化应激、感染和伤口愈合方面的潜在治疗应用，验证了传统医学实践，并为现代植物疗法中的林业副产品提供了一种可持续方法，可能有助于皮肤疾病的治疗。
