Yao Fangjie, Guan Fangnian, Duan Luyao, Long Li, Tang Hao, Jiang Yunfeng, Li Hao, Jiang Qiantao, Wang Jirui, Qi Pengfei, Kang Houyang, Li Wei, Ma Jian, Pu Zhien, Deng Mei, Wei Yuming, Zheng Youliang, Chen Xianming, Chen Guoyue
Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China.
State Key Laboratory of Crop Gene Exploitation and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, China.
Front Plant Sci. 2021 Dec 22;12:783830. doi: 10.3389/fpls.2021.783830. eCollection 2021.
Stripe rust (caused by f. sp. ) is one of the most severe diseases affecting wheat production. The disease is best controlled by developing and growing resistant cultivars. Chinese wheat () landraces have excellent resistance to stripe rust. The objectives of this study were to identify wheat landraces with stable resistance and map quantitative trait loci (QTL) for resistance to stripe rust from 271 Chinese wheat landraces using a genome-wide association study (GWAS) approach. The landraces were phenotyped for stripe rust responses at the seedling stage with two predominant Chinese races of f. sp. in a greenhouse and the adult-plant stage in four field environments and genotyped using the 660K wheat single-nucleotide polymorphism (SNP) array. Thirteen landraces with stable resistance were identified, and 17 QTL, including eight associated to all-stage resistance and nine to adult-plant resistance, were mapped on chromosomes 1A, 1B, 2A, 2D, 3A, 3B, 5A, 5B, 6D, and 7A. These QTL explained 6.06-16.46% of the phenotypic variation. Five of the QTL, , , , and , were likely new. Five Kompetitive allele specific PCR (KASP) markers for four of the QTL were converted from the significant SNP markers. The identified wheat landraces with stable resistance to stripe rust, significant QTL, and KASP markers should be useful for breeding wheat cultivars with durable resistance to stripe rust.
条锈病（由小麦条锈菌引起）是影响小麦生产的最严重病害之一。通过培育和种植抗病品种能最好地控制该病。中国小麦地方品种对条锈病具有优异抗性。本研究的目的是利用全基因组关联研究（GWAS）方法，从271份中国小麦地方品种中鉴定出具有稳定抗性的小麦地方品种，并定位抗条锈病的数量性状位点（QTL）。在温室中用两种中国小麦条锈菌的优势生理小种对这些地方品种进行苗期条锈病反应表型鉴定，并在四个田间环境中进行成株期表型鉴定，同时使用660K小麦单核苷酸多态性（SNP）芯片进行基因分型。鉴定出13个具有稳定抗性的地方品种，并在1A、1B、2A、2D、3A、3B、5A、5B、6D和7A染色体上定位了17个QTL，其中8个与全生育期抗性相关，9个与成株期抗性相关。这些QTL解释了6.06%-16.46%的表型变异。其中5个QTL，即、、、和，可能是新的。针对其中4个QTL的5个竞争性等位基因特异性PCR（KASP）标记由显著的SNP标记转化而来。鉴定出的对条锈病具有稳定抗性的小麦地方品种、显著的QTL和KASP标记，将有助于培育对条锈病具有持久抗性的小麦品种。
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