通过被动血凝试验检测血清中针对铜绿假单胞菌外毒素的抗体。
Serum antibody to Pseudomonas aeruginosa exotoxin measured by a passive hemagglutination assay.
作者信息
Pollack M, Taylor N S
出版信息
J Clin Microbiol. 1977 Jul;6(1):58-61. doi: 10.1128/jcm.6.1.58-61.1977.
Abstract
A passive hemagglutination (PHA) assay for antibody to Pseudomonas aeruginosa exotoxin is described which utilizes chromic chloride-treated ovine erythrocytes coated with purified toxin. PHA antitoxin titers correlated well with those obtained by a cytotoxicity neutralization assay (r = 0.91, P less than 0.001), whereas the PHA assay was four to eight times as sensitive. The mean serum PHA titer of 16 patients convalescing from recent pseudomonas infections (log2 = 9.4 +/- 3.9) was significantly higher (P less than 0.001) than that of 17 healthy controls (2,7 +/- 2.6), and fourfold or greater rises were demonstrated in 5 of 7 patients examined sequentially. The lower levels of PHA antibody in sera from 11 of 17 controls suggested the acquisition of antitoxin secondary to asymptomatic infection.
摘要
本文描述了一种用于检测抗铜绿假单胞菌外毒素抗体的被动血凝（PHA）试验，该试验利用经氯化铬处理的、包被有纯化毒素的绵羊红细胞。PHA抗毒素滴度与通过细胞毒性中和试验获得的滴度相关性良好（r = 0.91，P < 0.001），而PHA试验的敏感性则高4至8倍。16例近期铜绿假单胞菌感染康复患者的血清PHA平均滴度（log2 = 9.4 +/- 3.9）显著高于17例健康对照者（2.7 +/- 2.6）（P < 0.001），在7例连续检测的患者中，有5例显示滴度升高4倍或更高。17例对照者中有11例血清中PHA抗体水平较低，提示无症状感染后获得了抗毒素。
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本文引用的文献
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Purification and characterization of Pseudomonas aeruginosa exotoxin.铜绿假单胞菌外毒素的纯化与特性分析
Infect Immun. 1974 Jan;9(1):113-8. doi: 10.1128/iai.9.1.113-118.1974.
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A new rapid method for genetic typing of human immunoglobulins.一种用于人类免疫球蛋白基因分型的新快速方法。
J Immunol. 1968 Feb;100(2):274-9.
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Chromic chloride: a coupling reagent for passive hemagglutination reactions.氯化铬：一种用于被动血凝反应的偶联试剂。
J Immunol. 1967 Nov;99(5):859-66.
4
Neutralizing antibody to Pseudomonas aeruginosa exotoxin in human sera: evidence for in vivo toxin production during infection.人血清中针对铜绿假单胞菌外毒素的中和抗体：感染期间体内毒素产生的证据。
Infect Immun. 1976 Oct;14(4):942-7. doi: 10.1128/iai.14.4.942-947.1976.
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Pseudomonas aeruginosa exotoxin: purification by preparative polyacrylamide gel electrophoresis and the development of a highly specific antitoxin serum.铜绿假单胞菌外毒素：通过制备性聚丙烯酰胺凝胶电泳进行纯化及高效特异性抗毒素血清的研制
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Exotoxin production by clinical isolates of pseudomonas aeruginosa.铜绿假单胞菌临床分离株的外毒素产生情况。
Infect Immun. 1977 Mar;15(3):776-80. doi: 10.1128/iai.15.3.776-780.1977.
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