Henricks P A, van der Tol M E, Verhoef J
Scand J Immunol. 1985 Dec;22(6):721-5. doi: 10.1111/j.1365-3083.1985.tb01935.x.
The effects of influenza virus A (H3N2) on several functions of human polymorphonuclear leukocytes (PMN) were examined. Incubation of PMN with virus induced chemiluminescence, aggregation, and degranulation of the leukocytes. The amount of chemiluminescence generated increased from 1 X 10(6) to 6 X 10(6) cpm when 2.5 X 10(6) to 2 X 10(7) virus particles were added to 2.5 X 10(6) PMN. Maximal aggregation occurred within 2 min and the response depended on the amount of virus added to the PMN. Release of acid phosphatase by virus-treated PMN was 62 +/- 12% within 1 h compared with 7 +/- 7% by control PMN (P less than 0.005). Incubation of PMN with influenza virus resulted in a diminished phagocytic activity of the phagocytes. PMN from a patient with chronic granulomatous disease were similarly affected. It was thus concluded that the observed defect in phagocytic activity was not due to the reactive oxygen species generated by the PMN during incubation with virus.
研究了甲型流感病毒（H3N2）对人多形核白细胞（PMN）多种功能的影响。将PMN与病毒一起孵育可诱导白细胞的化学发光、聚集和脱颗粒。当向2.5×10⁶个PMN中加入2.5×10⁶至2×10⁷个病毒颗粒时，产生的化学发光量从1×10⁶增至6×10⁶cpm。最大聚集在2分钟内发生，且反应取决于加入PMN的病毒量。病毒处理的PMN在1小时内酸性磷酸酶的释放率为62±12%，而对照PMN为7±7%（P＜0.005）。将PMN与流感病毒一起孵育导致吞噬细胞的吞噬活性降低。慢性肉芽肿病患者的PMN也受到类似影响。因此得出结论，观察到的吞噬活性缺陷并非由于PMN在与病毒孵育期间产生的活性氧物质所致。
