Nouioui Imen, Gomez-Escribano Juan Pablo, Pötter Gabriele, Jando Marlen, Wolf Jacqueline, Neumann-Schaal Meina, Mast Yvonne
Leibniz-Institut DSMZ - German Collection of Microorganisms and Cell Cultures, Inhoffenstraße 7B, Braunschweig, 38124, Germany.
Braunschweig Integrated Centre of Systems Biology (BRICS), Rebenring 56, Braunschweig, 38106, Germany.
BMC Microbiol. 2025 Sep 18;25(1):577. doi: 10.1186/s12866-025-04290-z.
Streptomyces strains remain one of the most promising bacteria for novel drug discovery and are of general interest for pharmaceutical, biotechnological, and agricultural applications. They have been successfully used in agriculture to protect a number of crops, including wheat and rice, against fungal infections.
Streptomyces sp. Agncl-13 was isolated from the surface sterilised root nodule of Alnus glutinosa, Newcastle upon Tyne, UK, and subjected to polyphasic taxonomic analysis and genome mining for plant growth promoting genes. Phenotypic, genetic and genomic data distinguished strain Agncl-13 from Streptomyces species with validly published names. Whole cell hydrolysates of the strain were rich in LL-DAP. Strain Agncl-13 had diphosphatidylglycerol, phosphatidylinositol, phosphatidylethanolamine, aminolipids, a glycolipid, a glycophospholipid, phospholipids and unidentified lipids, as polar lipids; as well as iso-C anteiso-C, iso-C, C, iso-C, and anteiso-C as major fatty acids (> 5%), and MK-9(H) and MK-9(H) as predominant menaquinones (> 10%). Digital DNA-DNA hybridization values between the genomic sequence of strain Agncl-13 and its phylogenomic relatives were below the threshold of 70%. The average nucleotide identity scores were below the thresholds 95-96% (bacteria) and 96.7% (Streptomyces) for bacteria species demarcation, respectively. The strain had several gene clusters whose products are involved in plant growth promotion. It was able to fix nitrogen, solubilise phosphate, and produce siderophore and ACC deaminase. In addition, strain Agncl-13 contained several secondary metabolite biosynthetic gene clusters which had a low similarity confidence to known BCGs. The strain displayed antimicrobial activity against Escherichia coli ΔtolC, Proteus vulgaris, multidrug resistant Staphylococcus aureus, and Candida albicans.
Based on polyphasic taxonomic data, the strain Agncl-13 = DSM 118684 = CECT 31215 is considered as a novel species for which the name Streptomyces colwelliae sp. nov. is proposed. This study highlights the ecological features of strain Agncl-13, which might be used as biocontrol agent and biofertilizer for sustainable agriculture. Further research investigations are needed to confirm the potential application of the proposed novel type strain.
链霉菌菌株仍是新型药物发现中最具潜力的细菌之一，在制药、生物技术和农业应用方面具有广泛的研究价值。它们已成功应用于农业，用于保护包括小麦和水稻在内的多种作物免受真菌感染。
从英国泰恩河畔纽卡斯尔的黏性桤木表面消毒后的根瘤中分离出链霉菌Agncl-13，并对其进行了多相分类分析和促进植物生长基因的基因组挖掘。表型、遗传和基因组数据将菌株Agncl-13与已有效发表名称的链霉菌物种区分开来。该菌株的全细胞水解产物富含LL-二氨基庚二酸。菌株Agncl-13含有双磷脂酰甘油、磷脂酰肌醇、磷脂酰乙醇胺、氨基脂、糖脂、糖磷脂、磷脂和未鉴定的脂质作为极性脂质；以及异-C、anteiso-C、异-C、C、异-C和anteiso-C作为主要脂肪酸（>5%），以及MK-9（H）和MK-9（H）作为主要的甲基萘醌（>10%）。菌株Agncl-13的基因组序列与其系统发育相关菌株之间的数字DNA-DNA杂交值低于70%的阈值。细菌物种划分的平均核苷酸同一性得分分别低于细菌物种的95-96%（细菌）和96.7%（链霉菌）的阈值。该菌株有几个基因簇，其产物参与促进植物生长。它能够固氮、溶解磷酸盐，并产生铁载体和ACC脱氨酶。此外，菌株Agncl-13含有几个次生代谢物生物合成基因簇，与已知的生物合成基因簇相似度较低。该菌株对大肠杆菌ΔtolC、普通变形杆菌、多重耐药金黄色葡萄球菌和白色念珠菌具有抗菌活性。
基于多相分类数据，菌株Agncl-13 = DSM 118684 = CECT 31215被认为是一个新物种，提议将其命名为Streptomyces colwelliae sp. nov.。本研究突出了菌株Agncl-13的生态特征，其可作为可持续农业的生物防治剂和生物肥料。需要进一步的研究调查来证实所提议的新型菌株的潜在应用。
