Cvjetanović B, Mel D M, Felsenfeld O
Bull World Health Organ. 1970;42(4):499-507.
Streptomycin-dependent Salmonella typhi containing O and H antigens was administered as a live oral antityphoid vaccine to chimpanzees. Five animals served as controls; 5 others received the vaccine 4 times at 3-day intervals; 4 further animals were given 4 doses of vaccine at 3-day intervals together with streptomycin; and 1 animal received the 4 doses of vaccine and a daily dose of streptomycin. The individual vaccine doses varied between 36x10(9) and 82x10(9) organisms, totalling about 258x10(9)Salm. typhi per animal. The chimpanzees were challenged with 26x10(9) cells of the virulent Salm. typhi Ty2 strain 10 days after immunization and followed up bacteriologically, serologically and clinically. It was observed that after this very heavy challenge the immunized animals that had received streptomycin with the vaccine were protected to some degree against the infection and showed fewer symptoms. The animal that received vaccine and streptomycin daily did not develop the disease.The authors point out that, while the strain used may have potential usefulness for the protection of man, further studies are needed to confirm the innocuity of the vaccine, to reduce the strain's reversion to streptomycin-independence, and to determine the relative effectiveness of different immunization dosages and schedules.
含有O和H抗原的链霉素依赖型伤寒沙门氏菌作为口服减毒伤寒疫苗接种给黑猩猩。5只动物作为对照；另外5只动物每隔3天接种4次疫苗；还有4只动物每隔3天接种4剂疫苗并同时注射链霉素；1只动物接种4剂疫苗并每天注射链霉素。每剂疫苗的菌数在36×10⁹至82×10⁹之间，每只动物总共接种约258×10⁹个伤寒沙门氏菌。在免疫10天后，用26×10⁹个强毒伤寒沙门氏菌Ty2菌株攻击黑猩猩，并进行细菌学、血清学和临床随访。观察到，在这种非常强烈的攻击后，接种疫苗时同时注射链霉素的免疫动物在一定程度上受到保护，免受感染，症状也较少。每天接种疫苗和链霉素的动物没有发病。作者指出，虽然所用菌株可能对保护人类有潜在用途，但需要进一步研究以确认疫苗的安全性，减少菌株回复为链霉素非依赖型的可能性，并确定不同免疫剂量和方案的相对有效性。
