Mel D M, Cvjetanović B, Felsenfeld O
Bull World Health Organ. 1970;43(3):431-7.
In earlier studies in man it has been demonstrated that streptomycin-dependent shigellae, if given orally in 5 sufficiently large doses, confer a very high type-specific protection against bacillary dysentery.In the present study, 2 groups of Erythrocebus patas monkeys were immunized with live streptomycin-dependent Shigella flexneri 2a, and a third group was not vaccinated. One of the vaccinated groups was given streptomycin with each dose of the live vaccine. The animals that received streptomycin with the vaccine were shedding these organisms in their faeces for a significantly longer period than the animals that did not receive streptomycin. A week after the last dose of vaccine, the animals were challenged with virulent Sh. flexneri 2a organisms. All the control animals developed diarrhoea and excreted challenge organisms for an average of 9 days. None of the 9 immunized animals showed pathological changes nor symptoms of dysentery. On average, they excreted challenge organisms for only 2.3 days.Immunological tests confirmed the immunity of the vaccinated animals.This study shows that 3 doses of live oral streptomycin-dependent Shigella vaccine given together with streptomycin are at least as effective as immunization with 5 doses of the same vaccine without streptomycin. Oral application of streptomycin, therefore, seems to have had an enhancing effect on the immunizing property of live oral streptomycin-dependent enteric vaccines.
在早期的人体研究中已经证明，链霉素依赖型志贺氏菌如果口服足够大的剂量，能对细菌性痢疾提供非常高的型特异性保护。在本研究中，两组赤猴用活的链霉素依赖型福氏志贺氏菌2a进行免疫，第三组未接种疫苗。其中一组接种疫苗的动物在每次接种活疫苗时都给予链霉素。接受疫苗并同时接受链霉素的动物粪便中排出这些菌的时间明显长于未接受链霉素的动物。在最后一剂疫苗接种一周后，用有毒力的福氏志贺氏菌2a菌株对动物进行攻击。所有对照动物都出现腹泻，排出攻击菌的平均时间为9天。9只免疫动物均未出现病理变化或痢疾症状。它们排出攻击菌的平均时间仅为2.3天。免疫学检测证实了接种动物的免疫力。本研究表明，3剂口服活的链霉素依赖型志贺氏菌疫苗与链霉素一起使用至少与5剂不含链霉素的相同疫苗免疫效果一样好。因此，口服链霉素似乎对口服活的链霉素依赖型肠道疫苗的免疫特性有增强作用。
