• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

用于检测针对福氏志贺氏菌抗原的免疫球蛋白G和免疫球蛋白A抗体的酶联免疫吸附测定。

Enzyme-linked immunosorbent assay for immunoglobulin G and immunoglobulin A antibodies to Shigella flexneri antigens.

作者信息

Keren D F

出版信息

Infect Immun. 1979 May;24(2):441-8. doi: 10.1128/iai.24.2.441-448.1979.

DOI:10.1128/iai.24.2.441-448.1979
PMID:378853
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC414321/
Abstract

An enzyme-linked immunosorbent assay has been developed to detect class-specific antibodies to Shigella flexneri lipopolysaccharide antigens. This enzyme-linked immunosorbent assay system has been used to measure antibodies present in serum or intestinal secretions without further purification. It is considerably more sensitive than passive hemagglutination, allowing detection of as little as 1.3 ng of specific immunoglobulin G antibody per ml in immune sera. Optimal conditions for this assay are outlined in this report.

摘要

已开发出一种酶联免疫吸附测定法来检测针对福氏志贺氏菌脂多糖抗原的类特异性抗体。该酶联免疫吸附测定系统已用于测量血清或肠道分泌物中存在的抗体，无需进一步纯化。它比被动血凝反应灵敏得多，能够检测出免疫血清中每毫升低至1.3纳克的特异性免疫球蛋白G抗体。本报告概述了该测定法的最佳条件。

相似文献

1
Enzyme-linked immunosorbent assay for immunoglobulin G and immunoglobulin A antibodies to Shigella flexneri antigens.用于检测针对福氏志贺氏菌抗原的免疫球蛋白G和免疫球蛋白A抗体的酶联免疫吸附测定。
Infect Immun. 1979 May;24(2):441-8. doi: 10.1128/iai.24.2.441-448.1979.
2
Determination of humoral antibodies against Shigella flexneri 2a T32 (Istrati) by ELISA.通过酶联免疫吸附测定法检测针对福氏志贺菌2a T32（伊斯特拉蒂）的体液抗体。
Arch Roum Pathol Exp Microbiol. 1984 Jul-Dec;43(3-4):279-84.
3
Shigella lipopolysaccharide antibodies in pediatric populations.儿科人群中的志贺氏菌脂多糖抗体
Pediatr Infect Dis J. 1995 Oct;14(10):859-65. doi: 10.1097/00006454-199510000-00008.
4
Immunoglobulin G (IgG) and IgA subclass pattern of human antibodies to Shigella flexneri and Salmonella serogroup B and D lipopolysaccharide O antigens.人类针对福氏志贺菌以及沙门氏菌B和D血清群脂多糖O抗原的抗体的免疫球蛋白G（IgG）和IgA亚类模式。
Infect Immun. 1986 Jun;52(3):834-9. doi: 10.1128/iai.52.3.834-839.1986.
5
Serological response to plasmid-encoded antigens in children and adults with shigellosis.患志贺氏菌病的儿童和成人对质粒编码抗原的血清学反应。
Diagn Microbiol Infect Dis. 1988 Jun;10(2):75-80. doi: 10.1016/0732-8893(88)90043-0.
6
Widal-type serology using live antigen for diagnosis of Shigella flexneri dysentery.使用活抗原的维达氏血清学检测法用于诊断福氏志贺菌痢疾。
Eur J Clin Microbiol. 1986 Oct;5(5):540-2. doi: 10.1007/BF02017699.
7
Monoclonal antibodies specific for Shigella flexneri lipopolysaccharides: clones binding to type I and type III:6,7,8 antigens, group 6 antigen, and a core epitope.针对福氏志贺氏菌脂多糖的单克隆抗体：与I型和III型：6,7,8抗原、6组抗原及一个核心表位结合的克隆
Infect Immun. 1986 Jul;53(1):103-9. doi: 10.1128/iai.53.1.103-109.1986.
8
Comparison of enzyme-linked immunosorbent assay and passive hemagglutination method for quantification of antibodies to lipopolysaccharide and tetanus toxoid in rats.酶联免疫吸附测定法与被动血凝法对大鼠脂多糖和破伤风类毒素抗体定量的比较
Infect Immun. 1979 Jun;24(3):798-803. doi: 10.1128/iai.24.3.798-803.1979.
9
Detection of antibodies to Mycoplasma pulmonis by an enzyme-linked immunosorbent assay.通过酶联免疫吸附测定法检测肺支原体抗体。
Infect Immun. 1978 Oct;22(1):161-70. doi: 10.1128/iai.22.1.161-170.1978.
10
Titration of antibodies to salmonella O antigens by enzyme-linked immunosorbent assay.通过酶联免疫吸附测定法对沙门氏菌O抗原抗体进行滴定
Infect Immun. 1972 Nov;6(5):703-8. doi: 10.1128/iai.6.5.703-708.1972.

引用本文的文献

1
Trivalent outer membrane vesicles-based combination vaccine candidate induces protective immunity against Campylobacter and invasive non-typhoidal Salmonella in adult mice.三价外膜囊泡基组合疫苗候选物诱导成年小鼠对弯曲杆菌和侵袭性非伤寒沙门氏菌的保护性免疫。
Med Microbiol Immunol. 2024 Oct 15;213(1):21. doi: 10.1007/s00430-024-00805-z.
2
Development of a novel S. Typhi and Paratyphi A outer membrane vesicles based bivalent vaccine against enteric fever.研发一种新型的基于伤寒沙门氏菌和甲型副伤寒沙门氏菌外膜囊泡的两价疫苗，用于防治肠热病。
PLoS One. 2018 Sep 14;13(9):e0203631. doi: 10.1371/journal.pone.0203631. eCollection 2018.
3
Expression of plant sweet protein brazzein in the milk of transgenic mice.在转基因小鼠的乳汁中表达植物甜味蛋白 Brazzein。
PLoS One. 2013 Oct 14;8(10):e76769. doi: 10.1371/journal.pone.0076769. eCollection 2013.
4
Immunoglobulin G and M antibodies to pneumococcal polysaccharides detected by enzyme-linked immunosorbent assay.通过酶联免疫吸附测定法检测到的针对肺炎球菌多糖的免疫球蛋白G和M抗体。
Infect Immun. 1980 Feb;27(2):411-7. doi: 10.1128/iai.27.2.411-417.1980.
5
No impairment of local intestinal immune response to keyhole limpet haemocyanin in the absence of Peyer's patches.在没有派尔集合淋巴结的情况下，对钥孔戚血蓝蛋白的局部肠道免疫反应未受损。
Immunology. 1981 Mar;42(3):431-5.
6
Intestinal immunoglobulin A responses in rabbits to a Salmonella typhi strain harboring a Shigella sonnei plasmid.兔对携带宋内志贺氏菌质粒的伤寒沙门氏菌菌株的肠道免疫球蛋白A反应。
Infect Immun. 1982 Jul;37(1):387-9. doi: 10.1128/iai.37.1.387-389.1982.
7
Enzyme-linked immunosorbent assay for detection of antibody to Treponema hyodysenteriae antigens.用于检测猪痢疾密螺旋体抗原抗体的酶联免疫吸附测定。
J Clin Microbiol. 1982 Feb;15(2):249-52. doi: 10.1128/jcm.15.2.249-252.1982.
8
In vitro synthesis of antibody to specific bacterial lipopolysaccharide by peripheral blood mononuclear cells from patients with alcoholic cirrhosis.酒精性肝硬化患者外周血单个核细胞体外合成针对特定细菌脂多糖的抗体。
Immunology. 1981 May;43(1):177-82.
9
Role of antigen form in development of mucosal immunoglobin A response to Shigella flexneri Antigens.抗原形式在福氏志贺菌抗原黏膜免疫球蛋白A应答发育中的作用。
Infect Immun. 1981 Mar;31(3):1193-202. doi: 10.1128/iai.31.3.1193-1202.1981.
10
Comparison of ELISA and RIA for measurement of pneumococcal antibodies before and after vaccination with 14-valent pneumococcal capsular polysaccharide vaccine.14价肺炎球菌荚膜多糖疫苗接种前后，酶联免疫吸附测定法（ELISA）和放射免疫测定法（RIA）检测肺炎球菌抗体的比较
J Clin Pathol. 1981 Jan;34(1):93-8. doi: 10.1136/jcp.34.1.93.

本文引用的文献

1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
Enzyme-linked immunosorbent assay. II. Quantitative assay of protein antigen, immunoglobulin G, by means of enzyme-labelled antigen and antibody-coated tubes.酶联免疫吸附测定。二、借助酶标记抗原和抗体包被管对蛋白质抗原、免疫球蛋白G进行定量测定。
Biochim Biophys Acta. 1971 Dec 28;251(3):427-34. doi: 10.1016/0005-2795(71)90132-2.
3
Solid-phase radioimmunoassay in antibody-coated tubes.在抗体包被管中的固相放射免疫测定。
Science. 1967 Dec 22;158(3808):1570-2. doi: 10.1126/science.158.3808.1570.
4
Enzyme-linked immunosorbent assay (ELISA). Quantitative assay of immunoglobulin G.酶联免疫吸附测定（ELISA）。免疫球蛋白G的定量测定。
Immunochemistry. 1971 Sep;8(9):871-4. doi: 10.1016/0019-2791(71)90454-x.
5
Coupling of enzymes to proteins with glutaraldehyde. Use of the conjugates for the detection of antigens and antibodies.用戊二醛将酶与蛋白质偶联。偶联物用于抗原和抗体的检测。
Immunochemistry. 1969 Jan;6(1):43-52. doi: 10.1016/0019-2791(69)90177-3.
6
Titration of antibodies to salmonella O antigens by enzyme-linked immunosorbent assay.通过酶联免疫吸附测定法对沙门氏菌O抗原抗体进行滴定
Infect Immun. 1972 Nov;6(5):703-8. doi: 10.1128/iai.6.5.703-708.1972.
7
Protective capacity of antibodies against E. coli O antigen with special reference to the avidity.抗大肠杆菌O抗原抗体的保护能力，特别提及亲和力。
Int Arch Allergy Appl Immunol. 1974;46(3):470-80. doi: 10.1159/000231150.
8
Local antibody production in experimental pyelonephritis: amount, avidity, and immunoglobulin class.实验性肾盂肾炎中的局部抗体产生：数量、亲和力及免疫球蛋白类别
Infect Immun. 1974 Sep;10(3):411-5. doi: 10.1128/iai.10.3.411-415.1974.
9
The cross-linking of proteins with glutaraldehyde and its use for the preparation of immunoadsorbents.蛋白质与戊二醛的交联及其在免疫吸附剂制备中的应用。
Immunochemistry. 1969 Jan;6(1):53-66. doi: 10.1016/0019-2791(69)90178-5.
10
Determination of the optimal ammonium sulfate concentration for the fractionation of rabbit, sheep, horse, and goat antisera.确定用于兔、羊、马和山羊抗血清分级分离的最佳硫酸铵浓度。
Appl Microbiol. 1973 Jan;25(1):26-36. doi: 10.1128/am.25.1.26-36.1973.