用于检测针对福氏志贺氏菌抗原的免疫球蛋白G和免疫球蛋白A抗体的酶联免疫吸附测定。
Enzyme-linked immunosorbent assay for immunoglobulin G and immunoglobulin A antibodies to Shigella flexneri antigens.
作者信息
Keren D F
出版信息
Infect Immun. 1979 May;24(2):441-8. doi: 10.1128/iai.24.2.441-448.1979.
Abstract
An enzyme-linked immunosorbent assay has been developed to detect class-specific antibodies to Shigella flexneri lipopolysaccharide antigens. This enzyme-linked immunosorbent assay system has been used to measure antibodies present in serum or intestinal secretions without further purification. It is considerably more sensitive than passive hemagglutination, allowing detection of as little as 1.3 ng of specific immunoglobulin G antibody per ml in immune sera. Optimal conditions for this assay are outlined in this report.
摘要
已开发出一种酶联免疫吸附测定法来检测针对福氏志贺氏菌脂多糖抗原的类特异性抗体。该酶联免疫吸附测定系统已用于测量血清或肠道分泌物中存在的抗体，无需进一步纯化。它比被动血凝反应灵敏得多，能够检测出免疫血清中每毫升低至1.3纳克的特异性免疫球蛋白G抗体。本报告概述了该测定法的最佳条件。
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本文引用的文献
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