干扰素在MM病毒于L细胞中增殖的作用。
Role of interferon in the propagation of MM virus in L cells.
作者信息
Giron D J
出版信息
Appl Microbiol. 1969 Oct;18(4):584-8. doi: 10.1128/am.18.4.584-588.1969.
MM virus propagated in mouse brain replicates to low titers in L cells without production of cytopathic effect (CPE). After growing the virus in BHK-21 cells, however, the virus replicates to high titers in L cells with complete CPE. It was found that suspensions of MM virus propagated in L cells directly from the mouse brain contained much more interferon than did suspensions of virus which had first been grown in BHK-21 cells. Mouse brain suspensions of the virus were also found to contain high interferon titers. Treatment of L cells with actinomycin D before infection with mouse brain-grown virus resulted in full virus replication with CPE. BHK-21 cell-grown virus diluted in L cell interferon behaved like mouse brain-grown virus in L cells. It is concluded that the presence of interferon in the inoculum is largely responsible for the suppression of MM virus replication in L cells.
在小鼠脑中繁殖的MM病毒在L细胞中复制至低滴度，且不产生细胞病变效应（CPE）。然而，在BHK - 21细胞中培养该病毒后，它在L细胞中以高滴度复制并产生完全的CPE。研究发现，直接从鼠脑在L细胞中繁殖的MM病毒悬液比先在BHK - 21细胞中培养的病毒悬液含有更多的干扰素。还发现病毒的小鼠脑悬液也含有高干扰素滴度。在用鼠脑培养的病毒感染L细胞之前，用放线菌素D处理L细胞会导致病毒完全复制并产生CPE。在L细胞干扰素中稀释的BHK - 21细胞培养的病毒在L细胞中的表现与鼠脑培养的病毒相似。得出的结论是，接种物中干扰素的存在在很大程度上导致了MM病毒在L细胞中的复制受到抑制。
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