Morse J H, Morse S I
J Exp Med. 1970 Jun 1;131(6):1342-57. doi: 10.1084/jem.131.6.1342.
Two distinct particles have been recognized in the extracellular fluid of B. pertussis cultures. Both appeared to arise from the surface (cell wall) of the organism. One of these, a membranous particle, seemed to derive from long projections on the organism composed of the outer membrane of the cell wall. The second particle, a fine filament, was not readily comparable with any previously described bacterial structure. The two particles could be separated from each other by gradient centrifugation in CsCl. Lymphocytosis-promoting factor and histamine-sensitizing activity were only associated with fractions containing the fine filaments.
在百日咳博德特氏菌培养物的细胞外液中已识别出两种不同的颗粒。两者似乎都源自该生物体的表面(细胞壁)。其中一种是膜状颗粒,似乎源自该生物体上由细胞壁外膜组成的长突起。第二种颗粒是细纤维,与任何先前描述的细菌结构都不太容易比较。通过在氯化铯中进行梯度离心可以将这两种颗粒彼此分离。淋巴细胞增多促进因子和组胺致敏活性仅与含有细纤维的级分相关。