高比活度标记黄曲霉毒素的制备。
Preparation of labeled aflatoxins with high specific activities.
作者信息
Hsieh D P, Mateles R I
出版信息
Appl Microbiol. 1971 Jul;22(1):79-83. doi: 10.1128/am.22.1.79-83.1971.
Abstract
Resting cells of Aspergillus parasiticus ATCC 15517 were used to prepare highly labeled aflatoxins from labeled acetate. High synthetic activity in growing cells was evidenced only during 40 to 70 hr of incubation. Glucose was required for high incorporation efficiency, whereas the concentration of the labeled acetate determined the specific activity of the product. When labeled acetate was continuously added to maintain a concentration near but not exceeding 10 mm, in a culture containing 30 g of glucose per liter, 2% of its labels could be recovered in the purified aflatoxins which have a specific activity more than three times that of the labeled acetate.
摘要
寄生曲霉ATCC 15517的静止细胞被用于从标记的乙酸盐制备高度标记的黄曲霉毒素。仅在培养40至70小时期间，生长细胞中才显示出高合成活性。高掺入效率需要葡萄糖，而标记乙酸盐的浓度决定了产物的比活性。当在每升含有30克葡萄糖的培养物中连续添加标记乙酸盐以维持浓度接近但不超过10毫米时，其2%的标记物可在纯化的黄曲霉毒素中回收，这些黄曲霉毒素的比活性是标记乙酸盐的三倍以上。
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